MED16 Antibody

Western Blot: MED16 Antibody [NBP2-17255] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa C: HepG2 5% SDS PAGE NBP2-17255 diluted at 1:1000

• Reactivity: Hu, Mu, Rt, Xp
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MED16 Antibody Summary

• Description: Novus Biologicals Rabbit MED16 Antibody (NBP2-17255) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human MED16. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Mouse (97%), Rat (97%), Xenopus (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MED16
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (90%), Zebrafish (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for MED16 Antibody

• DRIP92thyroid hormone receptor associated protein 5
• mediator complex subunit 16THRAP5
• mediator of RNA polymerase II transcription subunit 16
• Thyroid hormone receptor-associated protein 5
• Thyroid hormone receptor-associated protein complex 95 kDa component
• thyroid hormone receptor-associated protein, 95-kD subunit
• TRAP95
• Vitamin D3 receptor-interacting protein complex 92 kDa component

Background

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MED16
• Uniprot:
  • Q9Y2X0(Human)