MED15 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: MED15 Antibody [NBP1-89018] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Immunohistochemistry-Paraffin: MED15 Antibody [NBP1-89018] - Staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MED15 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MED15 Antibody - BSA Free (NBP1-89018) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-MED15 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: QKLVSQIEDAMRKAGVAHSKSSKDMESHVFLKAKTRDEYLSLVARLIIHFRDIHNKKSQASVSDPMNALQSLTGGPAAGAAGIGMPPRGPGQSLGGMGSLGAMGQPMSLSGQP
• Predicted Species: Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MED15
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:20
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  MED15 Protein (NBP1-89018PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MED15 Antibody - BSA Free

• ARC105Arc105
• CAG7A
• CTG repeat protein 7a
• CTG7A
• FLJ42282
• FLJ42935
• mediator complex subunit 15PC2 glutamine/Q-rich-associated protein
• mediator of RNA polymerase II transcription subunit 15
• PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associatedprotein
• PC2-glutamine-rich-associated protein
• PCQAPDKFZp686A2214
• Positive cofactor 2 glutamine/Q-rich-associated protein
• positive cofactor 2, glutamine/Q-rich-associated protein
• TIG1DKFZp762B1216
• TIG-1TPA-inducible gene 1 protein
• TNRC7
• TPA inducible gene-1
• TPA inducible protein
• trinucleotide repeat containing 7,105-kD
• Trinucleotide repeat-containing gene 7 protein

Background

MED15 is encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MED15 Antibody (NBP1-89018)(1)

Publication using NBP1-89018

• Thiaville MM, Dudenhausen EE, Awad KS et al. Activated transcription via mammalian amino acid response elements does not require enhanced recruitment of the Mediator complex. Nucleic Acids Res 2008-10-01 [PMID: 18757893]

Bioinformatics

• Gene Symbol: MED15