MDM2/HDM2 Antibody (HDM2-323) - BSA Free

Western Blot: MDM2/HDM2 Antibody (HDM2-323) [NB600-1312] - Whole extracts of HEK-293 cells overexpressing human (lane 1) or mouse (lane 2) MDM2 were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-MDM2 Clone: HDM2-323. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: HDM2-323
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 2.0 mg/ml

MDM2/HDM2 Antibody (HDM2-323) - BSA Free Summary

• Description: Novus Biologicals Mouse MDM2/HDM2 Antibody (HDM2-323) - BSA Free (NB600-1312) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide conjugated to KLH, corresponding to amino acids 389-402 of Human MDM2.
• Epitope: The epitope recognized by the antibody is localized in the C-terminal region of MDM2 (amino acids 389-402 of human MDM2).
• Localization: Nuclear and cytoplasmic. Expressed predominantly in the nucleoplasm. Interaction with ARF (P14) results in the localisation of both proteins to the nucleus. The nucleolar localisation signals in both ARF and MDM2 may be necessary to allow efficient nucleo
• Specificity: MDM2 (HDM2-323)
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MDM2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1-2 ug/ml
• Application Notes: WB: Detects a band of approximately 90 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 2.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for MDM2/HDM2 Antibody (HDM2-323) - BSA Free

• ACTFS
• HDM2
• HDMX
• MDM2 oncogene, E3 ubiquitin protein ligase
• MDM2

Background

MDM2 is an E3 ubiquitin ligase that recognizes the N-terminal trans-activation domain (TAD) of the p53 transcription factor. MDM2 is a p53 responsive gene

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MDM2
• Entrez:
  • 4193(Human)
  • 17246(Mouse)