![Western Blot: MDM2/HDM2 Antibody (HDM2-323) [NB600-1312] - Whole extracts of HEK-293 cells overexpressing human (lane 1) or mouse (lane 2) MDM2 were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-MDM2 Clone: HDM2-323. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.](http://images.novusbio.com/fullsize/MDM2-HDM2-Antibody-HDM2-323-Western-Blot-NB600-1312-img0003.jpg "Western Blot: MDM2/HDM2 Antibody (HDM2-323) [NB600-1312] - Whole extracts of HEK-293 cells overexpressing human (lane 1) or mouse (lane 2) MDM2 were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-MDM2 Clone: HDM2-323. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA |
| Clone | HDM2-323 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 2.0 mg/ml |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to amino acids 389-402 of Human MDM2. |
| Epitope | The epitope recognized by the antibody is localized in the C-terminal region of MDM2 (amino acids 389-402 of human MDM2). |
| Localization | Nuclear and cytoplasmic. Expressed predominantly in the nucleoplasm. Interaction with ARF (P14) results in the localisation of both proteins to the nucleus. The nucleolar localisation signals in both ARF and MDM2 may be necessary to allow efficient nucleo |
| Specificity | MDM2 (HDM2-323) |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | MDM2 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Detects a band of approximately 90 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 10mM PBS (pH 7.4) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 2.0 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
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