MDM1 Overexpression Lysate (Native)

Images

 
Western Blot: MDM1 Overexpression Lysate (Adult Normal) [NBL1-12969] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MDM1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MDM1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205187 and based on accession number NM_017440. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Mdm1 nuclear protein homolog (mouse) (MDM1), transcript variant 1, mRNA.
Gene
MDM1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
80.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MDM1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MDM1 Overexpression Lysate (Native)

  • FLJ95264
  • Mdm1 nuclear protein homolog (mouse)
  • Mdm4, transformed 3T3 cell double minute 1, p53 binding protein (mouse)
  • Mdm4, transformed 3T3 cell double minute 1, p53 binding protein
  • nuclear protein double minute 1
  • nuclear protein MDM1

Background

This gene encodes a nuclear protein similar to the mouse double minute 1 protein. The mouse gene is located in double minute (DM) chromatin particles and is amplified in the mouse transformed 3T3 cell line, and the protein is able to bind to p53. In mouse several transcripts have been described for this gene which result from alternative polyadenylation, splicing and exon usage. Transcript Variant: This variant (1) encodes the longest isoform (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol MDM1