MBL1 Antibody (8G6)

Immunohistochemistry-Frozen: MBL1 Antibody (8G6) [NBP1-60127] - MBL-A (8G6) deposition in developing murine atherosclerotic lesions. Staining of frozen tissue sections with antibody 8G6. Anti-mouse MBL-A at 2ug/ml (2h, RT). MBL-A was detected on the intima to media border as well as throughout the media (insert). Furthermore, extensive MBL-A deposition was seen at sites of necrosis (upper right corner).

• Reactivity: Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 8G6
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

MBL1 Antibody (8G6) Summary

• Immunogen: mouse MBL-A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Mbl1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 2 ug/ml
  • Western Blot 1:100-1:2000
• Application Notes: In Western blots, non-reduced sample treatment was used. The band sizes were 191, 263 and 316 kDa (see Liu, H et al.). For IHC-F: Tissue sections were fixed in 4% PBS-buffered formaldehyde and pretreated with 2% hydrogen peroxide in methanol for 20 minutes at 4 degrees Celsius to quench endogenous peroxidases. (See Windbichler, M et al and Matthijsen, R et al)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS and 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Alternate Names for MBL1 Antibody (8G6)

• MBL1
• MBL-1
• MBL-A
• MBP-A
• p28b
• RaRF p28B
• S-MBP

Background

Mannose Binding Lectin (MBL), also called mannosebinding protein (MBP), is a calcium dependent oligomeric protein that belongs to the collectin family of proteins. It contains a collagen-like domain and a carbohydrate recognition domain enabling MBL to recognize carbohydrates (such as mannose and N-acetylglucosamine) on pathogens. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways, by using attached mannose binding lectin-associated serine proteases (MASP-2) in an antibody- and C1q-independent manner. MASP-2 permits cleavage of C4 and C2 to form a C3 convertase. Once it has bound, MBL exhibits complement-dependent antibacterial activities such as microbial opsonization and/or microbial lysis via membrane attack complexes and therefore plays an important role in innate immunity. In human, MBL is encoded by a single gene, whereas in mice there are two homologous proteins, termed MBL-A and MBL-C. The MBL-A concentration in serum is about 6-fold lower compared to that of MBL-C... MBL-A, but not MBL-C, was found to be an acute phase protein in casein and LPS-injection models. Moreover, it has been shown that MBL-A deficient mice have aberrant antigen-specific IgM responses and suffer from increased susceptibility to infection . Note that the monoclonal antibody 8G6 is a calcium-dependent antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MBL1 Antibody (NBP1-60127)(6)

Publications using NBP1-60127

• Matthijsen RA, de Winther MP, Kuipers D et al. Macrophage-specific expression of mannose-binding lectin controls atherosclerosis in low-density lipoprotein receptor-deficient mice. Circulation 2009-04-01 [PMID: 19380618]
• Abe Y, Kuroda Y, Kuboki N, Matsushita M, Yokoyama N, Kojima N. Contribution of complement component C3 and complement receptor type 3 to carbohydrate-dependent uptake of oligomannose-coated liposomes by peritoneal macrophages. J Biochem. 144(5):563-70. 2008-11-01 [PMID: 18694897]
• Held K, Thiel S, Loos M et al. Increased susceptibility of complement factor B/C2 double knockout mice and mannan-binding lectin knockout mice to systemic infection with Candida albicans. Mol Immunol. 2008-09-01 [PMID: 18672286]
• Petry F, Jakobi V, Wagner S, Tessema TS, Thiel S, Loos M. Binding and activation of human and mouse complement by Cryptosporidium parvum (Apicomplexa) and susceptibility of C1q- and MBL-deficient mice to infection. de Mol Immunol;45(12):3392-400. 2008-07-01 [PMID: 18501966]
• Windbichler M, Echtenacher B, Takahashi K, Ezekowitz RA, Schwaeble WJ, Jenseniuis JC, Mannel DN. Investigations on the involvement of the lectin pathway of complement activation in anaphylaxis. Int Arch Allergy Immunol;141(1):11-23. 2006-01-01 [PMID: 16804320]
• Liu H, Jensen L, Hansen S, Petersen SV, Takahashi K, Ezekowitz AB, Hansen FD, Jensenius JC, Thiel S. Characterization and quantification of mouse mannan-binding lectins (MBL-A and MBL-C) and study of acute phase responses. Scand J Immunol;53(5):489-97. 2001-05-01 [PMID: 11309157]

Bioinformatics

• Gene Symbol: Mbl1