Max Antibody (4E1.0-1A9)

Images

 
Immunocytochemistry/ Immunofluorescence: Max Antibody (4E10-1A9) [H00004149-M01] - Analysis of monoclonal antibody to MAX on HeLa cell. Antibody concentration 10 ug/ml.
Proximity Ligation Assay: Max Antibody (4E10-1A9) [H00004149-M01] - Analysis of protein-protein interactions between MAPK14 and MAX. HeLa cells were stained with anti-MAPK14 rabbit purified polyclonal 1:1200 and ...read more
ELISA: Max Antibody (4E1.0-1A9) [H00004149-M01] - Max Antibody (4E10-1A9) [H00004149-M01] - Detection limit for recombinant GST tagged MAX is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, PLA
Clone
4E1.0-1A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Max Antibody (4E1.0-1A9) Summary

Immunogen
MAX (AAH03525, 1 a.a. - 151 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSDNDDIEVESDADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS
Localization
Nuclear
Specificity
MAX - MYC associated factor X
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MAX
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Proximity Ligation Assay
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Control
Max Lysate (NBP2-65724)
Reviewed Applications
Read 1 Review rated 5
using
H00004149-M01 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Max Antibody (4E1.0-1A9)

  • BHLHD4
  • bHLHd4MGC11225
  • bHLHd5
  • bHLHd6
  • bHLHd7
  • bHLHd8
  • Class D basic helix-loop-helix protein 4
  • helix-loop-helix zipper protein
  • MAX protein
  • Max
  • MGC10775
  • MGC18164
  • MGC34679
  • MGC36767
  • MYC associated factor X
  • Myc-associated factor X
  • orf1
  • protein max

Background

The protein encoded by this gene is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature for some of them is unknown.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Max Antibody (H00004149-M01) (0)

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Review for Max Antibody (H00004149-M01) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00004149-M01:
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WB
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Images Ratings Applications Species Date Details
Western Blot Max H00004149-M01
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reviewed by:
Julien COUTIER
WB Human 08/30/2018
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Summary

ApplicationWestern Blot
Sample TestedHuman keratinocytes
SpeciesHuman
Lot07303-S1

Comments

CommentsTotal protein extract, 50 ug per lane, membrane blocked with PBS-BSA 3%, antibody diluted at 1/1000 in PBS overnight at 4°C.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Max Antibody (H00004149-M01) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Max Antibody (H00004149-M01)

Discover related pathways, diseases and genes to Max Antibody (H00004149-M01). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Max Antibody (H00004149-M01)

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Blogs on Max.

Dual applications of a c-Myc antibody in mitochondrial research
c-Myc, a proto-oncogene, has documented involvement in cellular differentiation, cell growth, cell death and tumor formation.  Target genes of the Myc family include those that participate in cell survival, translation, transcription, metabolism and...  Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g....  Read full blog post.

The c-Myc Antibody: A Major Tool in Cancer Research
C-Myc is a widely expressed transcription factor, regulating cellular differentiation, proliferation, cell cycle progression and pro-apoptotic gene expression. The c-Myc antibody is widely used in cancer research, as a number of human tumors have been...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Julien COUTIER
08/30/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol MAX
Entrez
OMIM
Uniprot