MAT1A Overexpression Lysate (Native)

Images

 
Western Blot: MAT1A Overexpression Lysate (Adult Normal) [NBL1-12909] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAT1A.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MAT1A Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203765 and based on accession number NM_000429. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens methionine adenosyltransferase I, alpha (MAT1A), mRNA.
Gene
MAT1A

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
43.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MAT1A

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MAT1A Overexpression Lysate (Native)

  • AdoMet synthase 1
  • adoMet synthetase 1
  • AMS1
  • MAT
  • MATA1MAT 1
  • MAT-I/III
  • Methionine adenosyltransferase 1
  • methionine adenosyltransferase I, alpha
  • Methionine adenosyltransferase I/III
  • S-adenosylmethionine synthase isoform type-1
  • S-adenosylmethionine synthetase isoform type-1
  • SAMS
  • SAMS1EC 2.5.1.6

Background

This gene catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for MAT1A Lysate (NBL1-12909) (0)

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Bioinformatics

Gene Symbol MAT1A