Human MAT1A ELISA Kit (Colorimetric)

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Tissue homogenates and other biological fluids
• Standard Curve Range: 0.625 - 40 ng/mL (example only; lot dependent)
• Sensitivity: 0.255 ng/mL (example only; lot dependent)

Human MAT1A ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 4.5 hours
• Standard Curve Range: 0.625 - 40 ng/mL (example only; lot dependent)
• Sensitivity: 0.255 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MAT1A

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human MAT1A ELISA Kit (Colorimetric)

• AdoMet synthase 1
• adoMet synthetase 1
• AMS1
• MAT
• MATA1MAT 1
• MAT-I/III
• Methionine adenosyltransferase 1
• methionine adenosyltransferase I, alpha
• Methionine adenosyltransferase I/III
• S-adenosylmethionine synthase isoform type-1
• S-adenosylmethionine synthetase isoform type-1
• SAMS
• SAMS1EC 2.5.1.6

Background

MAT1A and MAT2A are catalytic subunits of methionine adenosyltransferase (MAT), which catalyzes the formation of S-adenosyltransferase for methionine catabolism. Inactivation of MAT1A has been linked to liver disease. In addition, in certain liver cancers, expression of MAT1A is replaced by expression of MAT1B. Overexpression of MAT2A has also been observed in colon cancer, leading to the proposal that MAT2A facilitates cancer cell growth.

MAT1A and MAT2A antibodies are useful tools for cancer research and liver function research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for MAT1A ELISA Kit (NBP3-56248). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: MAT1A