MARCH6 Antibody

Western Blot: MARCH6 Antibody [NB100-93441] - Staining of Human Lung lysate with antibody at 0.2 ug/mL (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MARCH6 Antibody Summary

• Description: Novus Biologicals Goat MARCH6 Antibody (NB100-93441) is a polyclonal antibody validated for use in WB and ELISA. Anti-MARCH6 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QGRRVIFQKVKE corresponding to internal region according to NP_005876.2.
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MARCHF6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.2 - 0.5 ug/mL
• Application Notes: WB: Approx. 100 kDa band observed in human lung lysates (calculated MW of 103 kDa band according to NP_005876.2). An additional band of unknown identity was also consistently observed at 18 kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Theoretical MW: 103 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MARCH6 Antibody

• E3 ubiquitin-protein ligase MARCH6
• EC 6.3.2.-
• MARCH-VIDOA10
• membrane-associated ring finger (C3HC4) 6
• Membrane-associated RING finger protein 6
• Membrane-associated RING-CH protein VI
• Protein TEB-4
• RNF176KIAA0597Doa10 homolog
• TEB4RING finger protein 176

Background

MARCH6 (Membrane associated RING finger protein 6) belongs to the MARCH family, which contains at least seven membrane associated RING-CH (MARCH)proteins. MARCH proteins are E3 ubiquitin ligases and are located to subcellular membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MARCH6 Antibody (NB100-93441)(1)

Publication using NB100-93441

• Kreft SG, Wang L, Hochstrasser M. Membrane topology of the yeast endoplasmic reticulum-localized ubiquitin ligase Doa10 and comparison with its human ortholog TEB4 (MARCH-VI). J Biol Chem 2006-02-24 [PMID: 16373356]

Bioinformatics

• Gene Symbol: MARCHF6
• Entrez:
  • 10299(Human)
  • 223455(Mouse)
• Uniprot:
  • O60337(Human)