MARCH2 Antibody

Western Blot: MARCH2 Antibody [NBP2-21582] - Sample (30 ug of whole cell lysate) A: HepG2 membrane 12% SDS PAGE gel, diluted at 1:5000.

Product Discontinued

MARCH2 Antibody Summary

• Description: Novus Biologicals Rabbit MARCH2 Antibody (NBP2-21582) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human MARCH2. The exact sequence is proprietary.
• Localization: Endoplasmic reticulum membrane; Multi-pass membrane protein; Lysosome membrane; Multi-pass membrane protein; Endosome membrane; Multi-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MARCH2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:10000
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen shows the following sequence identity with these species: Mouse (94%), Rat (94%), Porcine (95%), Bovine (91%), Zebrafish (81%), Xenopus laevis (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for MARCH2 Antibody

• EC 6.3.2
• EC 6.3.2.-
• MARCH-IIHSPC240
• membrane-associated ring finger (C3HC4) 2
• Membrane-associated RING finger protein 2
• Membrane-associated RING-CH protein II
• RING finger protein 172
• RNF172E3 ubiquitin-protein ligase MARCH2

Background

MARCH2 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH2 reduces surface accumulation of several glycoproteins and appears to regulate early endosome-to-trans-Golgi network (TGN) trafficking (Bartee et al., 2004 [PubMed 14722266]; Nakamura et al., 2005 [PubMed 15689499]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MARCH2
• Uniprot:
  • Q9P0N8(Human)