MARCH2 Antibody

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Western Blot: MARCH2 Antibody [NBP2-21582] - Sample (30 ug of whole cell lysate) A: HepG2 membrane 12% SDS PAGE gel, diluted at 1:5000.

Product Details

Summary
Reactivity Hu, Rt, Po, BvSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

MARCH2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human MARCH2. The exact sequence is proprietary.
Localization
Endoplasmic reticulum membrane; Multi-pass membrane protein; Lysosome membrane; Multi-pass membrane protein; Endosome membrane; Multi-pass membrane protein
Predicted Species
Rat (94%), Porcine (95%), Bovine (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MARCH2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish (81%), Xenopus laevis (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MARCH2 Antibody

  • EC 6.3.2
  • EC 6.3.2.-
  • MARCH-IIHSPC240
  • membrane-associated ring finger (C3HC4) 2
  • Membrane-associated RING finger protein 2
  • Membrane-associated RING-CH protein II
  • RING finger protein 172
  • RNF172E3 ubiquitin-protein ligase MARCH2

Background

MARCH2 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH2 reduces surface accumulation of several glycoproteins and appears to regulate early endosome-to-trans-Golgi network (TGN) trafficking (Bartee et al., 2004 [PubMed 14722266]; Nakamura et al., 2005 [PubMed 15689499]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MARCH2
Uniprot