MAPKAP Kinase 3 Antibody - BSA Free

Western Blot: MAPKAP Kinase 3 Antibody [NBP3-03381] - Analysis of extracts from normal (control) and MAPKAPK3 knockout (KO) HeLa cells, using MAPKAP Kinase 3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Western Blot: MAPKAP Kinase 3 Antibody - Azide and BSA Free [MAPKAP Kinase 3] - Western blot analysis of various lysates using MAPKAP Kinase 3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 90s.

Immunocytochemistry/ Immunofluorescence: MAPKAP Kinase 3 Antibody - Azide and BSA Free [MAPKAP Kinase 3] - Immunofluorescence analysis of A549 cells using MAPKAP Kinase 3 Rabbit pAb . Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MAPKAP Kinase 3 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit MAPKAP Kinase 3 Antibody - BSA Free (NBP3-03381) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human MAPKAP Kinase 3 (NP_004626.1). MDGETAEEQGGPVPPPVAPGGPGLGGAPGGRREPKKYAVTDDYQLSKQVLGLGVNGKVLECFHRRTGQKCALKLLYDSPKARQEVDHHWQASGGPHIVCILDVYENMHHGKRCLLIIMECMEGGELFSRIQERGDQAFTEREAAEIMRDIGTAIQFLHSHNIAHRDVKPENLLYTSKEKDAVLKLTDFGFAKETTQNALQTPCYTPYYVAPEVLGPEKYDKSCDMWSLGVIMYILLCGFP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPKAPK3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot 1:500-1:2000
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for MAPKAP Kinase 3 Antibody - BSA Free

• 3PKEC 2.7.11.1
• Chromosome 3p kinase
• EC 2.7.11
• MAP kinase-activated protein kinase 3
• MAPK-activated protein kinase 3
• MAPKAP kinase 3
• MAPKAP33pK
• MAPKAPK-3
• mitogen-activated protein kinase-activated protein kinase 3

Background

MAPKAP Kinase 3 encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: MAPKAPK3