MAP3K8/Tpl2/COT Overexpression Lysate (Native)

Images

 
Western Blot: MAP3K8 Overexpression Lysate (Adult Normal) [NBL1-12859] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAP3K8.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MAP3K8/Tpl2/COT Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC211171 and based on accession number NM_005204. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens mitogen-activated protein kinase kinase kinase 8 (MAP3K8), mRNA.
Gene
MAP3K8

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
52.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MAP3K8/Tpl2/COT

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MAP3K8/Tpl2/COT Overexpression Lysate (Native)

  • Cancer Osaka thyroid oncogene
  • c-COT
  • cot (cancer Osaka thyroid) oncogene
  • COT
  • COTMEKK8
  • EC 2.7.11.25
  • EST
  • ESTF
  • ESTFLJ10486
  • Ewing sarcoma transformant
  • MAP3K8
  • mitogen-activated protein kinase kinase kinase 8
  • Proto-oncogene c-Cot
  • proto-oncogene serine/threoine protein kinase
  • Serine/threonine-protein kinase cot
  • TPL2
  • Tpl-2
  • Tumor progression locus 2
  • tumor progression locus-2

Background

This gene was identified by its oncogenic transforming activity in cells. The encoded protein is a member of the serine/threonine protein kinase family. This kinase can activate both the MAP kinase and JNK kinase pathways. This kinase was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This kinase was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. Studies of a similar gene in rat suggested the direct involvement of this kinase in the proteolysis of NF-kappaB1,p105 (NFKB1). This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for MAP3K8/Tpl2/COT Lysate (NBL1-12859) (0)

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Bioinformatics

Gene Symbol MAP3K8
Entrez