MAP1B [p Thr1265] Antibody [DyLight 405] - SuperBUGS

Product Discontinued

MAP1B [p Thr1265] Antibody [DyLight 405] - SuperBUGS Summary

• Immunogen: Phosphopeptide made to a C-terminal portion of rat MAP1B. [Swiss-Prot# P15205]
• Modification: p Thr1265
• Marker: Neuronal Marker
• Specificity: Phosphorylated Thr1265 on MAP1B (Thr1261 in rat).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAP1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Use in Monkey reported in scientific literature (PMID:22654911).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for MAP1B [p Thr1265] Antibody [DyLight 405] - SuperBUGS

• DKFZp686E1099
• DKFZp686F1345
• FLJ38954
• FUTSCH
• MAP-1B
• MAP5
• microtubule-associated protein 1B

Background

MAP1B (microtubule-associated protein 1B) is the major cytoskeletal constituent of developing nervous system wherein it plays a key role in neuronal migration as well as differentiation. MAP1B interacts with LC1, ANP32A, TIAM2, GAN, DAPK1, tubulin tyrosine TTL etc. and binds at least two tubulin subunits in the polymer which is critical in nucleating microtubule polymerization as well as in stabilizing the microtubules. MAP1B is one of the major phosphorylation substrates for GSK-3beta which phosphorylates it at Ser1260/Thr1265 in differentiating neurons, a molecular switch for MAP1B mediated regulation of microtubule dynamics in the growing axons and the growth cones. MAP1B acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation/membrane blebbing and also brings about tyrosination of alpha-tubulin in neuronal microtubules along with its essential role in synaptic maturation. MAP1B is downstream of various signalling pathways that stimulate neurite growth or direct growth cone pathfinding, e.g. laminin, netrins, neurotrophins, wingless/integration site, or that influence neuronal migration, e.g. reelin. MAP1B is implicated in a number of neurological disorders, such as fragile X syndrome and giant axonal neuropathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP1B