MAP1B [p Thr1265] Antibody [DyLight 550] - SuperBUGS

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Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, Fi, Re, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 550

MAP1B [p Thr1265] Antibody [DyLight 550] - SuperBUGS Summary

Immunogen
Phosphopeptide made to a C-terminal portion of rat MAP1B. [Swiss-Prot# P15205]
Modification
p Thr1265
Marker
Neuronal Marker
Specificity
Phosphorylated Thr1265 on MAP1B (Thr1261 in rat).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
Application Notes
This MAP1B [p Thr1265] antibody (also known as SuperBUGS) is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on frozen sections. In WB a band can be seen at ~320 kDa. Recommended Blocking Buffer for ICC/IF and IHC: 5% (v/v) normal horse serum, 5% (v/v) normal goat serum, 0.2% (w/v) L-lysine, 0.2% (v/v) Triton X-100 in PBS pH 7.2

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
320 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse, rat, chicken, Xenopus, fish and reptile.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MAP1B [p Thr1265] Antibody [DyLight 550] - SuperBUGS

  • DKFZp686E1099
  • DKFZp686F1345
  • FLJ38954
  • FUTSCH
  • MAP-1B
  • MAP5
  • microtubule-associated protein 1B

Background

phospho MAP1B antibody, also known as SuperBUGS, is useful for detecting phosphorylated MAP1B at Thr1265. MAP1B is a developmentally regulated microtubule associated phosphoprotein that regulates microtubule dynamics in growing axons and growth cones. It is phosphorylated by GSK3 beta at Ser1260 and Thr1265. MAP1B plays an important role in neurite growth, growth-cone function and neuronal migration. MAP1B is also implicated in a number of neurological disorders including giant axonal neuropathy and Fragile X syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional MAP1B Products

MAP1B NBP1-42827R

Bioinformatics Tool for MAP1B Antibody (NBP1-42827R)

Discover related pathways, diseases and genes to MAP1B Antibody (NBP1-42827R). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for MAP1B Antibody (NBP1-42827R)

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PTMs for MAP1B Antibody (NBP1-42827R)

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Research Areas for MAP1B Antibody (NBP1-42827R)

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Blogs on MAP1B.

Why LC3B Antibodies Make Ideal Autophagosomes Membrane Markers
The human form of microtubule-associated protein light chain 3 (LC3) is expressed as 3 splice variants; LC3A, LC3B and LC3C (He et al., 2003). LC3B is a subunit of the MAP1A and MAP1B microtubule-binding pr...  Read full blog post.

SuperBUGS in the Brain: MAP1B Antibodies & Neuronal Disease Research
Recently, we at Novus Biologicals added a new phospho-MAP1B (phosphorylated microtubule-associated protein 1B) antibody to our antibody database. MAP1B is a developmentally regulated phosphoprotein thought to be involved in the assembly of microtubule...  Read full blog post.

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Bioinformatics

Gene Symbol MAP1B