MAP1B [p Thr1265] Antibody [FITC] - SuperBUGS

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Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, Fi, Re, XpSpecies Glossary
Applications ICC/IF, IHC, IHC-Fr
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC

MAP1B [p Thr1265] Antibody [FITC] - SuperBUGS Summary

Immunogen
Phosphopeptide made to a C-terminal portion of rat MAP1B. [Swiss-Prot# P15205]
Modification
p Thr1265
Marker
Neuronal Marker
Specificity
Phosphorylated Thr1265 on MAP1B (Thr1261 in rat).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAP1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
Application Notes
This MAP1B [p Thr1265] antibody (also known as SuperBUGS) is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry on frozen sections. In WB a band can be seen at ~320 kDa. Recommended Blocking Buffer for ICC/IF and IHC: 5% (v/v) normal horse serum, 5% (v/v) normal goat serum, 0.2% (w/v) L-lysine, 0.2% (v/v) Triton X-100 in PBS pH 7.2

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
320 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse, rat, chicken, Xenopus, fish and reptile.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for MAP1B [p Thr1265] Antibody [FITC] - SuperBUGS

  • DKFZp686E1099
  • DKFZp686F1345
  • FLJ38954
  • FUTSCH
  • MAP-1B
  • MAP5
  • microtubule-associated protein 1B

Background

MAP1B (microtubule-associated protein 1B) is the major cytoskeletal constituent of developing nervous system wherein it plays a key role in neuronal migration as well as differentiation. MAP1B interacts with LC1, ANP32A, TIAM2, GAN, DAPK1, tubulin tyrosine TTL etc. and binds at least two tubulin subunits in the polymer which is critical in nucleating microtubule polymerization as well as in stabilizing the microtubules. MAP1B is one of the major phosphorylation substrates for GSK-3beta which phosphorylates it at Ser1260/Thr1265 in differentiating neurons, a molecular switch for MAP1B mediated regulation of microtubule dynamics in the growing axons and the growth cones. MAP1B acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation/membrane blebbing and also brings about tyrosination of alpha-tubulin in neuronal microtubules along with its essential role in synaptic maturation. MAP1B is downstream of various signalling pathways that stimulate neurite growth or direct growth cone pathfinding, e.g. laminin, netrins, neurotrophins, wingless/integration site, or that influence neuronal migration, e.g. reelin. MAP1B is implicated in a number of neurological disorders, such as fragile X syndrome and giant axonal neuropathy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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MAP1B NBP1-42827F

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Discover related pathways, diseases and genes to MAP1B Antibody (NBP1-42827F). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on MAP1B.

Why LC3B Antibodies Make Ideal Autophagosomes Membrane Markers
The human form of microtubule-associated protein light chain 3 (LC3) is expressed as 3 splice variants; LC3A, LC3B and LC3C (He et al., 2003). LC3B is a subunit of the MAP1A and MAP1B microtubule-binding pr...  Read full blog post.

SuperBUGS in the Brain: MAP1B Antibodies & Neuronal Disease Research
Recently, we at Novus Biologicals added a new phospho-MAP1B (phosphorylated microtubule-associated protein 1B) antibody to our antibody database. MAP1B is a developmentally regulated phosphoprotein thought to be involved in the assembly of microtubule...  Read full blog post.

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Bioinformatics

Gene Symbol MAP1B