Human MAN2B1 - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human MAN2B1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39800] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Standard Curve Range: 31.2 - 2000 pg/mL (example only; lot dependent)
• Sensitivity: 14.7 pg/mL (example only; lot dependent)

Human MAN2B1 - Ready-To-Use ELISA Kit (Colorimetric) Summary

• Description: The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
  Assay Length: 3 hours
• Standard Curve Range: 31.2 - 2000 pg/mL (example only; lot dependent)
• Sensitivity: 14.7 pg/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MAN2B1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human MAN2B1 - Ready-To-Use ELISA Kit (Colorimetric)

• alpha B, lysosomal
• EC 3.2.1
• EC 3.2.1.24
• LAMAN
• Lysosomal acid alpha-mannosidase
• MAN2B1
• MANB
• Mannosidase alpha class 2B member 1
• Mannosidase Alpha-B
• mannosidase, alpha, class 2B, member 1

Background

MAN2B1 encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: MAN2B1