MAGOH Antibody - Azide and BSA Free

Western Blot: MAGOH Antibody [NBP2-97072] - Anti-MAGOH rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 15 kDa (We are unsure as to the identity of these extra bands).

Immunoprecipitation: MAGOH Antibody [NBP2-97072] - MAGOH was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate 2 ul anti-MAGOH rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-MAGOH rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MAGOH Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human MAGOH (Accession#: NP_002361.1; Met1-Ile146)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAGOH
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunoprecipitation 4-6 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for MAGOH Antibody - Azide and BSA Free

• MAGOHAMAGOH1
• mago-nashi (Drosophila) homolog, proliferation-associated
• mago-nashi homolog, proliferation-associated (Drosophila)
• protein mago nashi homolog

Background

Drosophila that have mutations in their mago nashi (grandchildless) gene produce progeny with defects in germplasm assembly and germline development. This gene encodes the mammalian mago nashi homolog. In mammals, mRNA expression is not limited to the germ plasm, but is expressed ubiquitously in adult tissues and can be induced by serum stimulation of quiescent fibroblasts. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAGOH