MAGEA10 Antibody - BSA Free

Western Blot: MAGEA10 Antibody [NBP3-06272] - Anti-MAGEA10 rabbit polyclonal antibody at 1:500 dilutionLane A: HL60 Whole Cell LysateLane B: U-251 MGWhole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:41 kDaObserved band size:43 kDa(We are unsure as to the identity of these extra bands.)

Immunohistochemistry-Paraffin: MAGEA10 Antibody [NBP3-06272] - Immunochemical staining of human MAGEA10 in human placenta with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: MAGEA10 Antibody [NBP3-06272] - MAGEA10 was immunoprecipitated using:Lane A:0.5 mg U-251 MG Whole Cell Lysate4 uL anti-MAGEA10 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG.Primary antibody:Anti-MAGEA10 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 41 kDaObserved band size :45 kDa

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MAGEA10 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human MAGEA10 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAGEA10
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for MAGEA10 Antibody - BSA Free

• Cancer/testis antigen 1.10
• CT1.10member 10
• MAGE-10 antigen
• melanoma antigen family A, 10
• melanoma-associated antigen 10

Background

MAGEA10 is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAGEA10