Immunohistochemistry-Paraffin: MAGE 1 Antibody (SPM282) [NBP2-32871] - Formalin-fixed, paraffin-embedded human Testis stained with MAGE-1 Monoclonal Antibody (SPM282).
Flow (Intracellular): MAGE 1 Antibody (SPM282) [NBP2-32871] - An intracellular stain was performed on MG-63 cells with MAGE 1 Antibody [SPM282] NBP2-34754AF647 (blue) and a matched isotype control (orange). Cells were ...read more
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34754)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.
Human MAGEA1 full length recombinant protein (Uniprot: P43355)
Recognizes a protein of 42-46kDa, identified MAGEA1 (MAGE-1). This monoclonal antibody does not cross-react with MAGE-2, -3, -4, -6 -9, -10, -or -12 protein. Human malignant neoplasms carry rejection antigens that are recognized by the patients' autologous, tumor directed and specific, cytolytic, CD8+ T lymphocyte clones (CTL). The MAGE family of genes codes an important group of antigens. It was identified that melanomas and primary glial brain tumors express common melanoma associated antigens (MAAs). Because MAGEA1 is expressed on a significant proportion of human neoplasms of various histological types (melanoma, brain tumors of glial origin, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas) and not on normal tissues, the encoded antigen may serve as a marker of early detection and target for cancer immunotherapy.
Protein A or G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Read Publication using NBP2-32871 in the following applications:
melanoma antigen family A, 1 (directs expression of antigen MZ2-E)
melanoma-associated antigen 1
melanoma-associated antigen MZ2-E
The MAGE 1 gene is a member of the melanoma antigen encoding gene family. These genes encode for HLA-restricted tumor associated rejection antigens recognized by cytotoxic T lymphocytes. Some of these target antigens may be potentially useful for cancer specific immunotherapy. The expression of MAGE genes has been reported not only in melanoma but also in various other malignant tumors such as hepatocellular carcinoma and germ cell tumors.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our MAGEA1 Antibody (SPM282) and receive a gift card or discount.