MAGEA1 Antibody (MZ2E/838) [Janelia Fluor® 525]

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC, ICC/IF
• Clone: MZ2E/838
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 525

MAGEA1 Antibody (MZ2E/838) [Janelia Fluor® 525] Summary

• Immunogen: Recombinant human MAGEA1 protein (Uniprot: P43355)
• Localization: Cytoplasmic
• Specificity: Recognizes a protein of 42-46kDa, identified MAGEA1 (MAGE-1). This monoclonal antibody does not cross-react with other members of MAGE-family. Human malignant neoplasms carry rejection antigens that are recognized by the patients' autologous, tumor directed and specific, cytolytic, CD8+ T lymphocyte clones (CTL). The MAGE family of genes codes an important group of antigens. It was identified that melanomas and primary glial brain tumors express common melanoma associated antigens (MAAs). Because MAGEA1 is expressed on a significant proportion of human neoplasms of various histological types (melanoma, brain tumors of glial origin, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas) and not on normal tissues, the encoded antigen may serve as a marker of early detection and target for cancer immunotherapy.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAGEA1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for MAGEA1 Antibody (MZ2E/838) [Janelia Fluor® 525]

• Antigen MZ2-E
• Cancer/testis antigen 1.1
• cancer/testis antigen family 1, member 1
• CT1.1
• CT1.1melanoma antigen family A 1
• MAGE-1 antigen
• MAGE1
• MAGE1A
• MAGE1melanoma antigen MAGE-1
• MAGEA1
• MAGE-A1
• melanoma antigen family A, 1 (directs expression of antigen MZ2-E)
• melanoma-associated antigen 1
• melanoma-associated antigen MZ2-E
• MGC9326
• MZ2-E

Background

The MAGE 1 gene is a member of the melanoma antigen encoding gene family. These genes encode for HLA-restricted tumor associated rejection antigens recognized by cytotoxic T lymphocytes. Some of these target antigens may be potentially useful for cancer specific immunotherapy. The expression of MAGE genes has been reported not only in melanoma but also in various other malignant tumors such as hepatocellular carcinoma and germ cell tumors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAGEA1