MAGE 1 Antibody (SPM282)

Immunohistochemistry-Paraffin: MAGE 1 Antibody (SPM282) [NBP2-32871] - Formalin-paraffin human testis stained with MAGE-1 MAb (SPM282).

Flow (Intracellular): MAGE 1 Antibody (SPM282) [NBP2-32871] - An intracellular stain was performed on U2OS cells with MAGE 1 Antibody [SPM282] NBP2-34754AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image using the Alexa Fluor 647 Azide and BSA Free format of this antibody.

Immunohistochemistry-Paraffin: MAGE 1 Antibody (SPM282) [NBP2-32871] - Formalin-fixed, paraffin-embedded human Testis stained with MAGE-1 Monoclonal Antibody (SPM282).

Flow (Intracellular): MAGE 1 Antibody (SPM282) [NBP2-32871] - An intracellular stain was performed on MG-63 cells with MAGE 1 Antibody [SPM282] NBP2-34754AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image using the Azide and BSA Free format of this antibody.

• Reactivity: Hu, Rt, Ca
• Applications: Flow, Flow-IC, ICC/IF, IHC, IHC-P
• Clone: SPM282
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MAGE 1 Antibody (SPM282) Summary

• Immunogen: Human MAGE 1 full length recombinant protein
• Localization: Cytoplasmic
• Specificity: Recognizes a protein of 42-46kDa, identified as MAGE-1. This MAb does not cross-react with MAGE-2, -3, -4, -6 -9, -10, -or -12 protein. Human malignant neoplasms carry rejection antigens that are recognized by the patients' autologous, tumor directed and specific, cytolytic, CD8+ T lymphocyte clones (CTL). The MAGE family of genes codes an important group of antigens. It was identified that melanomas and primary glial brain tumors express common melanoma associated antigens (MAAs). Because MAGE-1 is expressed on a significant proportion of human neoplasms of various histological types (melanoma, brain tumors of glial origin, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas) and not on normal tissues, the encoded antigen may serve as a marker of early detection and target for cancer immunotherapy.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAGEA1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Flow (Intracellular)
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml (NBP2-34754).

Alternate Names for MAGE 1 Antibody (SPM282)

• Antigen MZ2-E
• Cancer/testis antigen 1.1
• cancer/testis antigen family 1, member 1
• CT1.1melanoma antigen family A 1
• MAGE-1 antigen
• MAGE1A
• MAGE1melanoma antigen MAGE-1
• melanoma antigen family A, 1 (directs expression of antigen MZ2-E)
• melanoma-associated antigen 1
• melanoma-associated antigen MZ2-E
• MGC9326

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAGEA1
• Uniprot:
  • P43355(Human)