MAD2L1-binding protein Overexpression Lysate (Native)


Western Blot: MAD2L1-binding protein Overexpression Lysate (Adult Normal) [NBL1-12787] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAD2L1-binding protein.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

MAD2L1-binding protein Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC223487 and based on accession number NM_001003690. The protein contains a C-terminal DDK tag.
Homo sapiens MAD2L1 binding protein (MAD2L1BP), transcript variant 1, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
34.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for MAD2L1-binding protein

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MAD2L1-binding protein Overexpression Lysate (Native)

  • Caught by MAD2 protein
  • CMT2MGC11282
  • dJ261G23.1
  • KIAA0110RP1-261G23.6
  • MAD2L1 binding protein
  • MAD2L1-binding protein


The protein encoded by this gene was identified as a binding protein of the MAD2 mitotic arrest deficient-like 1 (MAD2/MAD2L1). MAD2 is a key component of the spindle checkpoint that delays the onset of anaphase until all the kinetochores are attached to the spindle. This protein may interact with the spindle checkpoint and coordinate cell cycle events in late mitosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Gene Symbol MAD2L1BP