Lysyl tRNA synthetase Overexpression Lysate

Images

 
Western Blot: Lysyl tRNA synthetase Overexpression Lysate (Adult Normal) [NBL1-12123] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lysyl tRNA synthetase.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Lysyl tRNA synthetase Overexpression Lysate Summary

Description
Transient overexpression lysate of lysyl-tRNA synthetase (KARS), transcript variant 2 The lysate was created in HEK293T cells, using Plasmid ID RC200311 and based on accession number NM_005548. The protein contains a C-MYC/DDK Tag.
Gene
KARS1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
67.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Lysyl tRNA synthetase Overexpression Lysate

  • CMTRIB
  • EC 6.1.1
  • EC 6.1.1.6
  • KARS2
  • KIAA0070
  • KRS
  • lysine tRNA ligase
  • Lysine--tRNA ligase
  • lysRS
  • lysyl-tRNA synthetase

Background

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) lacks an internal exon in the 5' region, which results in an upstream AUG start codon, as compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Lysyl tRNA synthetase Lysate (NBL1-12123) (0)

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Other Available Formats

NBL1-12123

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Bioinformatics

Gene Symbol KARS1