Lysosomal Pro-X Carboxypeptidase/PRCP Overexpression Lysate (Native)

Images

 
Western Blot: PRCP Overexpression Lysate (Adult Normal) [NBL1-14732] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PRCP.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Lysosomal Pro-X Carboxypeptidase/PRCP Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200677 and based on accession number NM_005040. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens prolylcarboxypeptidase (angiotensinase C) (PRCP), transcript variant 1, mRNA.
Gene
PRCP

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
53.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Lysosomal Pro-X Carboxypeptidase/PRCP

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Lysosomal Pro-X Carboxypeptidase/PRCP Overexpression Lysate (Native)

  • Angiotensinase C
  • EC 3.4.16.2
  • HUMPCP
  • Lysosomal carboxypeptidase C
  • lysosomal Pro-X carboxypeptidase
  • PCPMGC2202
  • PRCP
  • Proline carboxypeptidase
  • prolylcarboxypeptidase (angiotensinase C)
  • prolylcarboxypeptidase isoform 1 preproprotein
  • Prolylcarboxypeptidase

Background

The protein encoded by this gene is a lysosomal prolylcarboxypeptidase, which cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]. Transcript Variant: This variant (1) lacks an alternate in-frame exon compared to variant 2. The resulting isoform (1) has the same N- and C-termini but is shorter compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Lysosomal Pro-X Carboxypeptidase/PRCP Lysate (NBL1-14732) (0)

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Bioinformatics

Gene Symbol PRCP
Entrez