Lysosomal Pro-X Carboxypeptidase/PRCP Antibody (860821) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: WB
• Clone: 860821
• Clonality: Monoclonal
• Host: Rat
• Conjugate: CoraFluor 1

Lysosomal Pro-X Carboxypeptidase/PRCP Antibody (860821) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Lysosomal Pro‑X Carboxypeptidase/PRCP
  Met1-His496
  Accession # P42785
• Specificity: Detects human Lysosomal Pro‑X Carboxypeptidase/PRCP in ELISAs.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: PRCP
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Lysosomal Pro-X Carboxypeptidase/PRCP Antibody (860821) [CoraFluor™ 1]

• Angiotensinase C
• EC 3.4.16.2
• HUMPCP
• Lysosomal carboxypeptidase C
• lysosomal Pro-X carboxypeptidase
• PCPMGC2202
• PRCP
• Proline carboxypeptidase
• prolylcarboxypeptidase (angiotensinase C)
• prolylcarboxypeptidase isoform 1 preproprotein
• Prolylcarboxypeptidase

Background

PRCP is encoded by this gene is a lysosomal prolylcarboxypeptidase, which cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRCP