Metadherin Antibody

Western Blot: LYRIC Antibody [H00092140-B01P] - Analysis of MTDH expression in human placenta.

Immunocytochemistry/Immunofluorescence: LYRIC Antibody [H00092140-B01P] - Analysis of purified antibody to MTDH on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: LYRIC Antibody [H00092140-B01P] - Analysis of MTDH expression in Jurkat.

Western Blot: LYRIC Antibody [H00092140-B01P] - Analysis of MTDH expression in transfected 293T cell line by MTDH polyclonal antibody. Lane 1: MTDH transfected lysate(64.02 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

Metadherin Antibody Summary

• Immunogen: MTDH (NP_848927, 1 a.a. - 582 a.a.) full-length human protein. MAARSWQDELAQQAEEGSARLREMLSVGLGFLRTELGLDLGLEPKRYPGWVILVGTGALGLLLLFLLGYGWAAACAGSRKKRRSPPRKREEAAAVPAAAPDDLALLKNLRSEEQKKKNRKKLSEKPKPNGRTVEVAEGEAVRTPQSVTAKQPPEIDKKNEKSKKNKKKSKSDAKAVQNSSRHDGKEVDEGAWETKISHREKRQQRKRDKVLTDSGSLDSTIPGIENTITVTTEQLTTASFPVGSKKNKGDSHLNVQVSNFKSGKGDSTLQVSSGLNENLTVNGGGWNEKSVKLSSQISAGEEKWNSVSPASAGKRKAEPSAWSQDTGDANTNGKDWGRSWSDRSIFSGIGSTAEPVSQSTTSDYQWDVSRNQPYIDDEWSGLNGLSSADPNSDWNAPAEEWGNWVDEERASLLKSQEPIPDDQKVSDDDKEKGEGALPTGKSKKKKKKKKKQGEDNSTAQDTEELEKEIREDLPVNTSKTRPKQEKAFSLKTISTSDPAEVLVKNSQPIKTLPPATSTEPSVILSKSDSDKSSSQVPPILQETDKSKSNTKQNSVPPSQTKSETSWESPKQIKKKKKARRET
• Specificity: MTDH - metadherin,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: MTDH
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Metadherin Antibody

• 3D3
• AEG1
• AEG-13D3/LYRIC
• AEG1LYRIC/3D3
• Astrocyte elevated gene-1 protein
• LYRIC
• LYRICMetastasis adhesion protein
• Lysine-rich CEACAM1 co-isolated protein
• Metadherin
• MTDH
• protein LYRIC

Background

LYRIC is a type II membrane protein, the precise role of which has yet to be defined. It is expressed in breast and prostate carcinoma and is a potential mediator of lung metastasis. It has also been found in mouse Purkinje neurons and cerebellum. In epithelial cells it is recruited to tight junctions (TJ) during the maturation of the TJ complexes. LYRIC co-localizes with beta-catenin in normal and tumour tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Metadherin Antibody (H00092140-B01P)(1)

Publication using H00092140-B01P

• Liang Y, Hu J, Li J et al. Epigenetic Activation of TWIST1 by MTDH Promotes Cancer Stem-like Cell Traits in Breast Cancer. Cancer Res 2015-09-01 [PMID: 26141861] (Human)

FAQs for Metadherin Antibody (H00092140-B01P). (Showing 1 - 1 of 1 FAQ).

1. Could you give me some information about the H00092140-B01P, i.e. the concentration and the volume of each vial?
   • The lab has informed me that for the current lot 09063, the concentration is 0.5ug/ul. It is 50 ug in 100 ul package for each vial.

Bioinformatics

• Gene Symbol: MTDH
• Entrez:
  • 92140(Human)
• Uniprot:
  • NP_848927(Human)