Lyp/PTPN22 Antibody (340113)

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Daudi human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Lyp Monoclonal Antibody (Catalog # MAB3428) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Lyp at approximately 108 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Lyp was detected in immersion fixed paraffin-embedded sections of human lymph node using 25 µg/mL Mouse Anti-Human Lyp Monoclonal Antibody (Catalog # MAB3428) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 340113
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Lyp/PTPN22 Antibody (340113) Summary

• Immunogen: E. coli-derived recombinant human Lyp
  Ser306-Ser684
  Accession # Q9Y2R2
• Specificity: Detects human Lyp in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPN22
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lyp/PTPN22 Antibody (340113)

• EC 3.1.3.48
• Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP
• Lymphoid phosphatase
• lymphoid-specific protein tyrosine phosphatase
• Lyp
• Lyp1
• Lyp2
• LyPTP
• PEP
• PEST-domain phosphatase
• protein tyrosine phosphatase, non-receptor type 22 (lymphoid)
• protein tyrosine phosphatase, non-receptor type 8
• PTPN22
• PTPN8 (former)
• PTPN8LYP1
• tyrosine-protein phosphatase non-receptor type 22

Background

Lymphoid phosphatase (Lyp), also called LyPTP, Protein Tyrosine Phosphatase, Nonreceptor-type 22 (PTPN22), and PEST-domain Phosphatase (PEP), dephosphorylates tyrosine residues in proteins. Found primarily in bone marrow and lymphoid cell lines, an R620W gain-of-function mutation in Lyp has been associated with susceptibility to autoimmune diseases such as type I diabetes, rheumatoid arthritis, and lupus erythematosus. Mature PEP knockout mice have an excess of CD8 positive T cells and an exaggerated antigen-induced proliferative response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lyp/PTPN22 Antibody (MAB3428)(1)

Publication using MAB3428

• Arechiga AF, Habib T, He Y, Zhang X, Zhang ZY, Funk A, Buckner JH Cutting edge: The PTPN22 allelic variant associated with autoimmunity impairs B cell signaling. J. Immunol., 2009-03-15;182(6):3343-7. 2009-03-15 [PMID: 19265110] (Western Blot, Human)

Review for Lyp/PTPN22 Antibody (MAB3428) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ELISA Human 02/20/2015

Summary

• Application: ELISA
• Sample Tested: See PMID 23219421
• Species: Human

Bioinformatics

• Gene Symbol: PTPN22
• Uniprot:
  • Q9Y2R2()