Lyp/PTPN22 Antibody (340113)

Images

 
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Daudi human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse ...read more
Lyp was detected in immersion fixed paraffin-embedded sections of human lymph node using 25 µg/mL Mouse Anti-Human Lyp Monoclonal Antibody (Catalog # MAB3428) overnight at 4 °C. Tissue was stained with the Anti-Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
340113
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Lyp/PTPN22 Antibody (340113) Summary

Immunogen
E. coli-derived recombinant human Lyp
Ser306-Ser684
Accession # Q9Y2R2
Specificity
Detects human Lyp in Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PTPN22
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB3428 in the following applications:

Publications
Read Publication using
MAB3428 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lyp/PTPN22 Antibody (340113)

  • EC 3.1.3.48
  • Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP
  • Lymphoid phosphatase
  • lymphoid-specific protein tyrosine phosphatase
  • Lyp
  • Lyp1
  • Lyp2
  • LyPTP
  • PEP
  • PEST-domain phosphatase
  • protein tyrosine phosphatase, non-receptor type 22 (lymphoid)
  • protein tyrosine phosphatase, non-receptor type 8
  • PTPN22
  • PTPN8 (former)
  • PTPN8LYP1
  • tyrosine-protein phosphatase non-receptor type 22

Background

Lymphoid phosphatase (Lyp), also called LyPTP, Protein Tyrosine Phosphatase, Nonreceptor-type 22 (PTPN22), and PEST-domain Phosphatase (PEP), dephosphorylates tyrosine residues in proteins. Found primarily in bone marrow and lymphoid cell lines, an R620W gain-of-function mutation in Lyp has been associated with susceptibility to autoimmune diseases such as type I diabetes, rheumatoid arthritis, and lupus erythematosus. Mature PEP knockout mice have an excess of CD8 positive T cells and an exaggerated antigen-induced proliferative response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lyp/PTPN22 Antibody (MAB3428)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Filter By Species
Human
(1)
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Review for Lyp/PTPN22 Antibody (MAB3428) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB3428:
Filter by Applications
ELISA
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
ELISA Human 02/20/2015
View

Summary

ApplicationELISA
Sample TestedSee PMID 23219421
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23219421

Details

Detection NotesSee PMID 23219421

Comments

CommentsIndependent review from antibodyresource.com

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Lyp/PTPN22 Antibody (MAB3428) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for Lyp/PTPN22 Antibody (MAB3428)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Anonymous
02/20/2015
Application: ELISA
Species: Human

Bioinformatics

Gene Symbol PTPN22
Entrez
Uniprot