Recombinant Human Lyn Protein

Product Discontinued

Recombinant Human Lyn Protein Summary

• Description: Recombinant protein for Human Lyn
  
  

  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(BC059394)

• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: LYN
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: 180 nmol phosphate incorporated into Poly(Glu-Tyr) per minute per mg protein at 30C for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).
• Theoretical MW: 85 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
• Concentration: 0.1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Lyn Protein

• EC 2.7.10
• EC 2.7.10.2
• FLJ26625
• Hck-2
• JTK8
• Lyn
• tyrosine-protein kinase Lyn
• v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
• v-yes-1
• Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog

Background

Lyn is a 56 kd tyrosine kinase that is similar to mouse T-lymphocyte-specific tyrosine kinase p56lck and the v-yes protein as well as to the gene products of v-fgr and v-src. Northern hybridization analysis showed that a 3.2-kilobase Lyn mRNA was expressed in a variety of tissues of the human fetus (1). Lyn is expressed preferentially in B cells and can be coimmunoprecipitated with IgM suggesting that Lyn is physically associated with membrane-bound IgM, and participates in antigen-mediated signal transduction (2). Crosslinking of membrane-bound IgM with antibody induces rapid increase in activities of Lyn and Lyn-associated phosphatidylinositol 3-kinase (3). Crosslinking of B-cell antigen receptor also induces association of Lyn with an 85-kDa noncatalytic subunit of phosphatidylinositol 3-kinase. Thus, Lyn is functionally associated with membrane-bound IgM and participates in B-cell antigen receptor-mediated signaling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: LYN