Lyn Antibody

Western Blot: Lyn Antibody [NB100-93453] - (0.1ug/ml) staining of U937 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Lyn Antibody Summary

• Description: Novus Biologicals Goat Lyn Antibody (NB100-93453) is a polyclonal antibody validated for use in WB and ELISA. Anti-Lyn Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KTQPVRNTERT corresponding to internal region according to NP_002341.1.
• Epitope: C-KTQPVRNTERT
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LYN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 60 kDa band observed in lysates of U937 (calculated MW of 58.6 kDa band according to NP_002341.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Lyn Antibody

• EC 2.7.10
• EC 2.7.10.2
• FLJ26625
• Hck-2
• JTK8
• Lyn
• tyrosine-protein kinase Lyn
• v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
• v-yes-1
• Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog

Background

Src is the human homolog of the v-Src gene of the Rous sarcoma virus, also known as avian sarcoma virus or ASV. Src is the first proto-oncogenic non-receptor tyrosine kinase characterized in human. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility and adhesion. Src-family kinases contain an amino-terminal cell membrane anchor followed by an SH3 domain and an SH2 domain involved in modular association and activation, respectively. Src-family kinases are normally maintained in an inactive state and can be activated transiently during cellular events such as mitosis. The human Lyn gene maps to chromosome 8q13 and encodes a 505 amino acid protein. Hematopoietic tissues predominantly express Lyn, which influences normal immunoglobulin production and regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lyn Antibody (NB100-93453)(1)

Publication using NB100-93453

• Okamoto M, Hayakawa F, Miyata Y et al. Lyn is an important component of the signal transduction pathway specific to FLT3/ITD and can be a therapeutic target in the treatment of AML with FLT3/ITD. Leukemia 2007-03-01 [PMID: 17230226]

Bioinformatics

• Gene Symbol: LYN
• Entrez:
  • 4067(Human)
• Uniprot:
  • NP_002341.1(Human)