Lyn Antibody (2H8D7)

Western Blot: Lyn Antibody (2H8D7) [NB110-60538] - Analysis using LYN mouse mAb agains HL60 (1), L540 (2), SLLP-M2 (3), SEM (4) and Ramos (5) cell lysate.

Product Discontinued

Lyn Antibody (2H8D7) Summary

• Immunogen: Purified recombinant fragment of LYN expressed in E. Coli.
• Specificity: LYN (2H8D7)
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LYN
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Lyn Antibody (2H8D7)

• EC 2.7.10
• EC 2.7.10.2
• FLJ26625
• Hck-2
• JTK8
• Lyn
• tyrosine-protein kinase Lyn
• v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
• v-yes-1
• Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog

Background

Lyn (also known as p53/56 Lyn) is a membrane-associated protein tyrosine kinase (PTK) mostly expressed in hemopoietic cells which is important in cellular signaling. It contains an SH2 and SH3 domain and has been found to be cleaved after activation of caspases in apoptosis. A member of the Src family of PTKs, there are two known isoforms for Lyn which plays an indispensable role in the Fc epsilon RI (Fcer1) and the B-cell IgM receptor signaling pathway and is essential for Syk activation and Lat phosphorylation after Fcer1 aggregation and can also phosphor-ylate Tec on multiple residues. Lyn can also be regulated by IL-2 and IL-3.Lyn is a member of the src family of non-receptor protein tyrosine kinases that is predominantly expressed in haematopoietic tissues. Like all members of the src family, lyn is thought to participate in signal transduction from cell surface receptors that lack intrinsic tyrosine kinase activity. It is associated with a number of cell surface receptors including the B cell antigen receptor and immunoglobulin E receptor (FceRI).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Lyn Antibody (NB110-60538). (Showing 1 - 1 of 1 FAQ).

1. What is the concentration of this antibody?
   • This antibody is unpurified from ascites, so the concentration is unknown.

Bioinformatics

• Gene Symbol: LYN
• Entrez:
  • 4067(Human)