Ly6K Antibody

Western blot shows lysates of human testis tissue. PVDF Membrane was probed with 0.25 µg/mL of Sheep Anti-Human Ly6K Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6648) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Ly6K at approximately 25-27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Ly6K was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Sheep Anti-Human Ly6K Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6648) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Ly6K Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Ly6K
  Asp18-Gly138
  Accession # Q17RY6
• Specificity: Detects human Ly6K in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: LY6K
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ly6K Antibody

• cancer/testis antigen 97
• CO16
• CT97
• FLJ35226
• HSJ001348
• Ly6K
• ly-6K
• lymphocyte antigen 6 complex, locus K
• lymphocyte antigen 6K

Background

Ly6K (Lymphocyte antigen 6 locus K; also CO16) is a 26-27 kDa member of the Ly-6 antigen family of molecules. It has restricted expression, being described in testis and skin. Ly6K is found in/on carcinomas, and is known to circulate in normal and tumor-patient blood. Mature human Ly6K is a 121 amino acid (aa) GPI-linked (presumed) glycoprotein. It is synthesized as a 165 aa preproprecursor that contains a 17 aa signal sequence, a 121 aa mature region (aa 18-138), and a 27 aa C‑terminal propeptide. There are two potential splice variants, one that shows a 48 aa substitution, and another that shows a 33 aa substitution for aa 73-165. It is not clear if mouse Ly6K is a true molecular ortholog of human Ly6K. In any event, over aa 18-138, human Ly6K shares 39% aa identity with mouse Ly6K.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ly6K Antibody (AF6648)(5)

Publications using AF6648

• S Park, D Park, S Han, GE Chung, S Soh, HI Ka, HJ Joo, Y Yang LY6K depletion modulates TGF-beta and EGF signaling Cancer Medicine, 2023-04-19;0(0):. 4/19/2023 [PMID: 37076981]
• S Benti, PB Tiwari, DW Goodlett, L Daneshian, GB Kern, MD Smith, A Uren, M Chruszcz, LS Shimizu, G Upadhyay Small Molecule Binds with Lymphocyte Antigen 6K to Induce Cancer Cell Death Cancers (Basel), 2020-02-22;12(2):. 2020-02-22 [PMID: 32098321] (Western Blot, Human)
• Schiza C, Korbakis D, Jarvi K et al. Identification of TEX101-associated Proteins Through Proteomic Measurement of Human Spermatozoa Homozygous for the Missense Variant rs35033974 Molecular & Cellular Proteomics 2/1/2019 [PMID: 30429210]
• Schiza C, Korbakis D, Jarvi K et al. Identification of TEX101-associated Proteins Through Proteomic Measurement of Human Spermatozoa Homozygous for the Missense Variant rs35033974 Molecular & Cellular Proteomics 2019-02-01 [PMID: 30429210]
• AlHossiny M, Luo L, Frazier W, Steiner N, Gusev Y, Kallakury B, Glasgow E, Creswell K, Madhavan S, Kumar R, Upadhyay G Ly6E/K Signaling to TGFbeta Promotes Breast Cancer Progression, Immune Escape, and Drug Resistance. Cancer Res, 2016-04-11;76(11):3376-86. 2016-04-11 [PMID: 27197181] (IHC, Western Blot, Human)

Bioinformatics

• Gene Symbol: LY6K
• Uniprot:
  • Q17RY6(Human)