Ly6K Antibody (750747) [Unconjugated]

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 750747
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Ly6K Antibody (750747) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Ly6K
  Met1-Gly138
  Accession # Q17RY6
• Specificity: Detects human Ly6K in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: LY6K
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
• Control:

  Ly6K Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ly6K Antibody (750747) [Unconjugated]

• cancer/testis antigen 97
• CO16
• CT97
• FLJ35226
• HSJ001348
• Ly6K
• ly-6K
• lymphocyte antigen 6 complex, locus K
• lymphocyte antigen 6K

Background

Ly6K (Lymphocyte antigen 6 locus K; also CO16) is a 26-27 kDa member of the Ly-6 antigen family of molecules. It has restricted expression, being described in testis and skin. Ly6K is found in/on carcinomas, and is known to circulate in normal and tumor-patient blood. Mature human Ly6K is a 121 amino acid (aa) GPI-linked (presumed) glycoprotein. It is synthesized as a 165 aa preproprecursor that contains a 17 aa signal sequence, a 121 aa mature region (aa 18-138), and a 27 aa C‑terminal propeptide. There are two potential splice variants, one that shows a 48 aa substitution, and another that shows a 33 aa substitution for aa 73-165. It is not clear if mouse Ly6K is a true molecular ortholog of human Ly6K. In any event, over aa 18-138, human Ly6K shares 39% aa identity with mouse Ly6K.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Ly6K Antibody (MAB6648) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous Flow Human 06/09/2016

Summary

• Application: Flow Cytometry
• Sample Tested: human HEK293T cells overexoressed with Ly6K
• Species: Human
• Lot: CGRB0114091

Blocking

• Blocking Details: 2%FBS in PBS for 20 min on ice

Primary Anitbody

• Dilution Ratio: 1:100 diluted, 1 million cells incubted with antibody in 100ul on ice for 30 min in dark

Secondary Antibody

• Secondary Description: Biolegend Alexa Fluor 647 Goat anti-mouse IgG
• Secondary Manufacturer Cat#: biolegend cat# 405322
• Secondary Concentration: 1ul/flow reaction

Details

• Detection Notes: read by flow cytometry
• Fixation Details: no fixation, living cells were stained for surface protein expression
• Permeabilization Details: no permeabilization steps, cells were stained alive to detect surface expression
• Wash Description: 2%FBS in PBS once

Comments

• Comments: This antibody works very well in over-expressed cell line model. The over expression of Ly6K has also been confirmed by western blot.

Bioinformatics

• Gene Symbol: LY6K
• Uniprot:
  • Q17RY6(Human)