Ly6K Antibody (750747) [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
750747
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Ly6K Antibody (750747) [Unconjugated] Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Ly6K
Met1-Gly138
Accession # Q17RY6
Specificity
Detects human Ly6K in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
LY6K
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
Control
Ly6K Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
MAB6648 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ly6K Antibody (750747) [Unconjugated]

  • cancer/testis antigen 97
  • CO16
  • CT97
  • FLJ35226
  • HSJ001348
  • Ly6K
  • ly-6K
  • lymphocyte antigen 6 complex, locus K
  • lymphocyte antigen 6K

Background

Ly6K (Lymphocyte antigen 6 locus K; also CO16) is a 26-27 kDa member of the Ly-6 antigen family of molecules. It has restricted expression, being described in testis and skin. Ly6K is found in/on carcinomas, and is known to circulate in normal and tumor-patient blood. Mature human Ly6K is a 121 amino acid (aa) GPI-linked (presumed) glycoprotein. It is synthesized as a 165 aa preproprecursor that contains a 17 aa signal sequence, a 121 aa mature region (aa 18-138), and a 27 aa C‑terminal propeptide. There are two potential splice variants, one that shows a 48 aa substitution, and another that shows a 33 aa substitution for aa 73-165. It is not clear if mouse Ly6K is a true molecular ortholog of human Ly6K. In any event, over aa 18-138, human Ly6K shares 39% aa identity with mouse Ly6K.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ly6K Antibody (MAB6648) (0)

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Review for Ly6K Antibody (MAB6648) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB6648:
Filter by Applications
Flow
(1)
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(1)
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Images Ratings Applications Species Date Details
Flow Cytometry Ly6K MAB6648
Enlarge
5
reviewed by:
Anonymous
Flow Human 06/09/2016
View

Summary

ApplicationFlow Cytometry
Sample Testedhuman HEK293T cells overexoressed with Ly6K
SpeciesHuman
LotCGRB0114091

Blocking

Blocking Details2%FBS in PBS for 20 min on ice

Primary Anitbody

Dilution Ratio1:100 diluted, 1 million cells incubted with antibody in 100ul on ice for 30 min in dark

Secondary Antibody

Secondary DescriptionBiolegend Alexa Fluor 647 Goat anti-mouse IgG
Secondary Manufacturer Cat#biolegend cat# 405322
Secondary Concentration1ul/flow reaction

Details

Detection Notesread by flow cytometry
Fixation Detailsno fixation, living cells were stained for surface protein expression
Permeabilization Detailsno permeabilization steps, cells were stained alive to detect surface expression
Wash Description2%FBS in PBS once

Comments

CommentsThis antibody works very well in over-expressed cell line model. The over expression of Ly6K has also been confirmed by western blot.

Product General Protocols

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FAQs for Ly6K Antibody (MAB6648) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB6648U
Alexa Fluor 405 FAB6648V
Alexa Fluor 488 FAB6648G
Alexa Fluor 594 FAB6648T
Alexa Fluor 647 FAB6648R
Alexa Fluor 700 FAB6648N
Alexa Fluor 750 FAB6648S
Allophycocyanin FAB6648A

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Bioinformatics Tool for Ly6K Antibody (MAB6648)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
06/09/2016
Application: Flow
Species: Human

Bioinformatics

Gene Symbol LY6K
Uniprot