Ly6K Antibody (750747) [Allophycocyanin]

HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Ly6K APC-conjugated Monoclonal Antibody (Catalog # FAB6648A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 750747
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

Ly6K Antibody (750747) [Allophycocyanin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Ly6K
  Met1-Gly138
  Accession # Q17RY6
• Specificity: Detects human Ly6K in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: LY6K
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ly6K Antibody (750747) [Allophycocyanin]

• cancer/testis antigen 97
• CO16
• CT97
• FLJ35226
• HSJ001348
• Ly6K
• ly-6K
• lymphocyte antigen 6 complex, locus K
• lymphocyte antigen 6K

Background

Ly6K (Lymphocyte antigen 6 locus K; also CO16) is a 26-27 kDa member of the Ly-6 antigen family of molecules. It has restricted expression, being described in testis and skin. Ly6K is found in/on carcinomas, and is known to circulate in normal and tumor-patient blood. Mature human Ly6K is a 121 amino acid (aa) GPI-linked (presumed) glycoprotein. It is synthesized as a 165 aa preproprecursor that contains a 17 aa signal sequence, a 121 aa mature region (aa 18-138), and a 27 aa C‑terminal propeptide. There are two potential splice variants, one that shows a 48 aa substitution, and another that shows a 33 aa substitution for aa 73-165. It is not clear if mouse Ly6K is a true molecular ortholog of human Ly6K. In any event, over aa 18-138, human Ly6K shares 39% aa identity with mouse Ly6K.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LY6K
• Uniprot:
  • Q17RY6(Human)