Ly-6A/E Antibody (D7)

Product Discontinued

Ly-6A/E Antibody (D7) Summary

• Immunogen: IL-2-dependent mouse T-cell line (CTL-L)
• Specificity: Ly-6A/E (D7)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: LY6A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.25 microgram per one million cells in 100 microliter volume. Immunohistochemistry-frozen sections. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for Ly-6A/E Antibody (D7)

• Ly 6A/E
• Ly6A.2
• Ly6A/E
• Ly6E.1
• Lymphocyte antigen 6 complex locus A
• Lymphocyte antigen 6 complex locus E
• Retinoic acid induced gene E
• RIGE
• SCA1
• SCA-1
• SCA2
• Stem cell antigen 1
• Stem cell antigen 2
• T cell activating protein
• TAP
• Thymic shared antigen 1
• TSA1

Background

Ly-6A/E is an 18 kD member of the Ly-6 multigene family also known as Sca-1. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses. The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ly-6A/E Antibody (NB100-77513)(1)

Publication using NB100-77513

• van Bragt,MPA, N Ciliberti, WL Stanford et al. LY6A/E (SCA-1) expression in the mouse testis. Biol Reprod 2005-06-03 [PMID: 15930324] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: LY6A
• Entrez:
  • 110454(Mouse)