LTK Antibody

Western Blot: LTK Antibody [NB100-56425] - Analysis of LTK in human K562 cell lysate using this antibody at 1 ug/ml.

Product Discontinued

LTK Antibody Summary

• Immunogen: A portion of amino acids 800-864 of human LTK were used as the immunogen.
• Specificity: The amino acid sequence used as immunogen has 100% identity in human isoforms 1 and 2, and 81% homologous in mouse isoforms A, B, C, and D, and rat. LTK is an 864 amino acid protein with an approximate molecular weight of 94 kD.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LTK
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-2 ug/ml
• Control:

  K-562 Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for LTK Antibody

• EC 2.7.10.1
• leukocyte receptor tyrosine kinase
• leukocyte tyrosine kinase receptor
• leukocyte tyrosine kinase
• LTK
• TYK1
• TYK1Protein tyrosine kinase 1

Background

Leukocyte tyrosine kinase (LTK) is a receptor-type protein tyrosine kinase, belonging to the insulin receptor superfamily, and is mainly expressed in B lymphocyte precursors and neuronal tissues. LTK utilizes insulin receptor substrate-1 (IRS-1) and Shc as major two substrates and possesses two NPXY motifs for them separately, tyrosine 485 of one NPXY motif at the juxta membrane domain for IRS-1 and tyrosine 862 of another NPXY motif at the carboxyl-terminal domain for Shc. It has also been shown that p85 subunit of PI3 kinase directly binds to tyrosine 753 of LTK, which is located within a YXXM motif. Tyrosine 485 and 753 are responsible for IRS-1 and PI3K mediated survival effects of LTK, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LTK
• Entrez:
  • 4058(Human)
• Uniprot:
  • P29376()