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LSM1 Antibody

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Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-LSM1 )
Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-LSM1 )

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated

Order Details

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LSM1 Antibody Summary

Immunogen
The immunogen for this antibody was a sequence from 1a.a. to 133a.a. from LSM1(a 133a.a. long protein)
Epitope
Binds specifically to the 3'-terminal U-tract of U6 snRNA.
Specificity
LSM1
Clonality
Polyclonal
Host
Chicken
Gene
LSM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000. Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.
Control
LSM1 Overexpression Lysate

Reactivity Notes

This antibody is reactive against Human, mouse, rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for LSM1 Antibody

  • Cancer-associated Sm-like
  • CASMYJL124C
  • LSM1 homolog, U6 small nuclear RNA associated (S. cerevisiae)
  • Small nuclear ribonuclear CaSm
  • U6 snRNA-associated Sm-like protein LSm1

Background

SM-like proteins were identified in a variety of organisms based on sequence homology with the SM protein family. SM-like proteins contain the SM sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The SM-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB200-191
Immunocytochemistry/Immunofluorescence: DDX6 Antibody [NB200-191] - In normal condition, cells were fixed and stained for DDX6, that usually punctuate in process bodies (PBs). Primary Antibody rabbit-anti-DDX6 diluted 1:1000. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: DDX6 Antibody [NB200-191] - Section of human colon carcinoma (left) and mouse renal cell carcinoma(right). Antibody: Affinity purified rabbit anti-DDX6 used at a dilution of 1:200 (1ug/ml). Detection: DAB.
Immunoprecipitation: DDX6 Antibody [NB200-191] - Detection of human DDX6 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX6 antibody NB200-191 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP. DDX6 was also immunoprecipitated using rabbit anti-DDX6 antibody NB200-192, but not with 'DDX6 Ab', which is to another epitope from DDX6. Normal rabbit IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 1 second (A & B).
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Applications: WB, ICC/IF, IHC, IHC-P, IP
SLC36A1 Antibody
NBP2-80524
SLC36A1 Antibody
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Applications: WB, IHC, IHC-P
APPBP2 Antibody
NBP2-15432
Western Blot: APPBP2 Antibody [NBP2-15432] - Sample (30 ug of whole cell lysate) A: Jurkat 7.5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: APPBP2 Antibody [NBP2-15432] - Analysis of methanol-fixed HeLa, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: APPBP2 Antibody [NBP2-15432] - Paraffin-embedded Ca922 xenograft, using antibody at 1:100 dilution.
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Applications: WB, ICC/IF, IHC, IHC-P
DCP2 Antibody
NBP2-16109
Western Blot: DCP2 Antibody [NBP2-16109] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: DCP2 Antibody [NBP2-16109] - Mouse testis. DCP2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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ACE/CD143 Antibody
[Unconjugated]
AF1513
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ACE/CD143 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE/CD143 at approximately 218 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.
ACE/CD143 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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Applications: WB, Simple Western, Flow, IHC, IP, CyTOF-ready
DCP1B Antibody
NBP1-82018
Western Blot: DCP1B Antibody [NBP1-82018] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/Immunofluorescence: DCP1B Antibody [NBP1-82018] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: DCP1B Antibody [NBP1-82018] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
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H00080122-M01
Immunocytochemistry/Immunofluorescence: YSK4 Antibody (2A4) [H00080122-M01] - Analysis of monoclonal antibody to YSK4 on HeLa cell. Antibody concentration 40 ug/ml.
ELISA: YSK4 Antibody (2A4) [H00080122-M01] - Detection limit for recombinant GST tagged YSK4 is 1 ng/ml as a capture antibody.
(1 Publication)
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Splicing factor,
arginine/serine-rich ...
NBP1-87381
Western Blot: Splicing factor, arginine/serine-rich 11 Antibody [NBP1-87381] - Analysis in control (vector only transfected HEK293T lysate) and SRSF11 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: Splicing factor, arginine/serine-rich 11 Antibody [NBP1-87381] - Staining of human cell line U-2 OS shows localization to nuclear speckles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Splicing factor, arginine/serine-rich 11 Antibody [NBP1-87381] - Staining of human pancreas shows moderate to strong nuclear positivity in exocrine glandular cells.
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FKBP51/FKBP5
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Western Blot: FKBP51/FKBP5 Antibody [NBP1-84676] - Analysis using Anti-FKBP5 antibody NBP1-84676 (A) shows similar pattern to independent antibody NBP1-84677 (B).
Immunocytochemistry/Immunofluorescence: FKBP51/FKBP5 Antibody [NBP1-84676] - Staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.
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Ets-1 Antibody
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NBP2-53385
Immunohistochemistry-Paraffin: Ets-1 Antibody (ETS1/1801) [NBP2-53385] - Formalin-fixed, paraffin-embedded human Spleen stained with ETS1 Monoclonal Antibody (ETS1/1282).
SDS-Page: Ets-1 Antibody (ETS1/1801) [NBP2-53385]
Protein Array: Ets-1 Antibody (ETS1/1801) [NBP2-53385] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Ets-1 Antibody (ETS1/1801) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.
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IFIT2 Antibody
NBP2-15180
Western Blot: IFIT2 Antibody [NBP2-15180] - Analysis of IFIT2 in human K562 lysate in the 1) absence and 2) presence of immunizing peptide, and 3) mouse RAW lysate probed with 4 ug/ml of IFIT2 antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunocytochemistry/Immunofluorescence: IFIT2 Antibody [NBP2-15180] - SK-MEL-28 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-IFIT2 at 10 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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Western Blot: NONO Antibody [NB100-1556] - B-cell whole cell lysate 40ug. Image submitted by a verified customer review
Immunocytochemistry: NONO Antibody [NB100-1556] - NONO in human fibroblasts at 1:200 dilution in 1X PBS/1% BSA/0.3% Triton X-100, 16 hours 4C. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: NONO Antibody [NB100-1556] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- NONO used at a dilution of 1:1,000 (0.2 ug/ml) and 1:200 (1ug/ml). Detection: DAB
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Xrn1 Antibody
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Western Blot: Xrn1 Antibody [NB500-191] - Whole cell lysate (50 ug) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit antiXRN1 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: Xrn1 Antibody [NB500-191] - Detection of human XRN1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-XRN1 antibody NB500-191 (lot 3) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. XRN1 was also immunoprecipitated by a previous lot of this antibody (lot 2). Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
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NBP2-67121
Western Blot: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of Argonaute 2 on different lysates using anti-Argonaute 2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7
Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Staining Argonaute 2 in AGS cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (JF0992) [NBP2-67121] - Analysis of paraffin-embedded mouse stomach tissue using anti-Argonaute 2 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
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PATL1 Antibody
NBP1-93808
Immunocytochemistry/Immunofluorescence: PATL1 Antibody [NBP1-93808] - Staining of human cell line A-431 shows localization to cytoplasmic bodies. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PATL1 Antibody [NBP1-93808] - Staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.
(1 Publication)
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LSM7 Antibody
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Immunocytochemistry/Immunofluorescence: LSM7 Antibody [NBP1-92084] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: LSM7 Antibody [NBP1-92084] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus duct.
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Nocturnin Antibody
NBP2-37856
Immunocytochemistry/Immunofluorescence: Nocturnin Antibody [NBP2-37856] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: Nocturnin Antibody [NBP2-37856] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
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CCR4 Antibody
(205410) [Unconjugate
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CNOT6 Antibody
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Immunocytochemistry/Immunofluorescence: CNOT6 Antibody [NBP1-86564] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CNOT6 Antibody [NBP1-86564] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
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LSM1 Antibody
NB100-75412
LSM1 Antibody
Species: Hu, Mu, Rt
Applications: WB

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Control Lysate(s)

LSM1 Overexpression Lysate

Secondary Antibodies

 

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Bioinformatics

Gene Symbol LSM1
Entrez
Uniprot