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LSM1 Antibody

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Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-LSM1 )
Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. ( anti-LSM1 )

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Chicken
Conjugate
Unconjugated

Order Details

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LSM1 Antibody Summary

Immunogen
The immunogen for this antibody was a sequence from 1a.a. to 133a.a. from LSM1(a 133a.a. long protein)
Epitope
Binds specifically to the 3'-terminal U-tract of U6 snRNA.
Specificity
LSM1
Clonality
Polyclonal
Host
Chicken
Gene
LSM1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000. Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.
Control
LSM1 Overexpression Lysate

Reactivity Notes

This antibody is reactive against Human, mouse, rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Alternate Names for LSM1 Antibody

  • Cancer-associated Sm-like
  • CASMYJL124C
  • LSM1 homolog, U6 small nuclear RNA associated (S. cerevisiae)
  • Small nuclear ribonuclear CaSm
  • U6 snRNA-associated Sm-like protein LSm1

Background

SM-like proteins were identified in a variety of organisms based on sequence homology with the SM protein family. SM-like proteins contain the SM sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The SM-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunoprecipitation: DDX6 Antibody [NB200-191] - Detection of human DDX6 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX6 antibody NB200-191 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP. DDX6 was also immunoprecipitated using rabbit anti-DDX6 antibody NB200-192, but not with 'DDX6 Ab', which is to another epitope from DDX6. Normal rabbit IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 1 second (A & B).
Immunohistochemistry-Paraffin: DDX6 Antibody [NB200-191] - Section of human colon carcinoma (left) and mouse renal cell carcinoma(right). Antibody: Affinity purified rabbit anti-DDX6 used at a dilution of 1:200 (1ug/ml). Detection: DAB.
Immunocytochemistry/Immunofluorescence: DDX6 Antibody [NB200-191] - In normal condition, cells were fixed and stained for DDX6, that usually punctuate in process bodies (PBs). Primary Antibody rabbit-anti-DDX6 diluted 1:1000. Image submitted by a verified customer review.
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Western Blot: APPBP2 Antibody [NBP2-81781] - Analysis of APPBP2 in human brain tissue lysate with APPBP2 antibody at (A) 0.5 and (B) 1 ug/ml.
Immunohistochemistry-Paraffin: APPBP2 Antibody [NBP2-81781] - APPBP2 in human brain tissue with APPBP2 antibody at 5 ug/mL.
Immunocytochemistry/Immunofluorescence: APPBP2 Antibody [NBP2-81781] - Immunofluorescence of APPBP2 in human brain tissue with APPBP2 antibody at 20 ug/mL.
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Western Blot: DCP2 Antibody [NBP2-16109] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: DCP2 Antibody [NBP2-16109] - Zebrafish blastula cells expressing GFP-Rbpms2 fusions. Wild-type GFP-Rbpms2b localizes near the nucleus (top row), is apparently associated with the centrosome/spindle in some cells. (Top row) A subset of GFP-Rbpms2b positive granules are positive for the p-body marker Dcp2. (Second row) GFP-Rbpms2bsa9329 localization to the centrosome/spindle but not granules. (Third rod) GFP-Rbpms2bae32 and (Final row) GFP-Rbpms2aae30 localization. Insets show magnified views of the highlighted cells. Images are representative slices from Z-stacks of sphere stage embryos viewed from the animal pole. Image collected and cropped by CiteAb from the following publication (dx.plos.org/10.1371/journal.pgen.1007489) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: DCP2 Antibody [NBP2-16109] - Mouse testis. DCP2 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ACE/CD143 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE/CD143 at approximately 218 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.
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Immunohistochemistry-Paraffin: DCP1B Antibody [NBP1-82018] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunocytochemistry/Immunofluorescence: DCP1B Antibody [NBP1-82018] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.
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Western Blot: NONO Antibody [NB100-1556] - B-cell whole cell lysate 40ug. Image submitted by a verified customer review
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Immunocytochemistry: NONO Antibody [NB100-1556] - NONO in human fibroblasts at 1:200 dilution in 1X PBS/1% BSA/0.3% Triton X-100, 16 hours 4C. Image submitted by a verified customer review.
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Immunoprecipitation: Xrn1 Antibody [NB500-191] - Detection of human XRN1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XRN1 antibody NB500-191 (lot NB500-191-4) used for IP at 6 ug per reaction. XRN1 was also immunoprecipitated by a previous lot of this antibody (lot NB500-191-3). For blotting immunoprecipitated XRN1, NB500-191 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
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Immunocytochemistry/Immunofluorescence: PATL1 Antibody [NBP1-93808] - Staining of human cell line A-431 shows localization to cytoplasmic bodies. Antibody staining is shown in green.
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Immunohistochemistry-Paraffin: LSM7 Antibody [NBP1-92084] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferus duct.
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Immunocytochemistry/Immunofluorescence: Nocturnin Antibody [NBP2-37856] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
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(205410) [Unconjugate
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Immunocytochemistry/Immunofluorescence: CNOT6 Antibody [NBP1-86564] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.
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Control Lysate(s)

LSM1 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LSM1
Entrez
Uniprot