This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.
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FAQs for LRRK2 Peptide (NB300-268PEP). (Showing 1 - 1 of 1 FAQs).
Can you provide us with a dilution this Peptide should be used at? Is their a recommendation concerning BSA or Milk powder blocking when using this peptide in combination with its appropriate antibody?
Our LRRK2 Peptide NB300-268PEP is designed to be used as a blocking peptide for NB300-268. We recommend using the peptide anywhere between 50-200 molar excess. We also recommend doing a titer to ensure proper blocking. We typically incubate the peptide with the antibody in PBS or TBS for 1 hour at room temperature with gentle shaking prior to incubation with the membrane. We have not noticed any interaction of the peptide with milk or BSA once antibody has been incubated with the peptide before incubating in a blocking buffer. Our blocking buffer contains 1% BSA, 5% non-fat dry milk in TBST.
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