LRRC32/GARP Antibody (Plato-1) - BSA Free

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IP
• Clone: Plato-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

LRRC32/GARP Antibody (Plato-1) - BSA Free Summary

• Immunogen: Recombinant human LRRC32 (aa 20-627).
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LRRC32
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunoprecipitation 1:200
  • Western Blot 1:1000
• Application Notes: NBP1-97653 is >95% pure by SDS-PAGE. It detects a band of ~100kDa (Fc Fusion) by Western blot, native protein is ~72kDa. 1 test means: 1ul of MAb is used to stain 500,000 cells in a sample volume of 50ul.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for LRRC32/GARP Antibody (Plato-1) - BSA Free

• D11S833E
• D11S833Eleucine-rich repeat-containing protein 32
• GARP
• GARPGarpin
• Garpin
• Glycoprotein A repetitions predominantgarpin
• leucine rich repeat containing 32
• LRRC32

Background

LRRC32 (leucine rich repeat containing 32; also known as GARP or Garpin; Glycoprotein A repetitions predominant) is a glycoprotein expressed on the cell surface of megakaryocytes, platelets and activated regulatory T (Treg) cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LRRC32/GARP Antibody (NBP1-97653)(3)

Publications using NBP1-97653

• Wang R, Kozhaya L, Mercer F et al. Expression of GARP selectively identifies activated human FOXP3+ regulatory T cells. Proc Natl Acad Sci U S A. 2009-08-01 [PMID: 19666573]
• Tran DQ, Andersson J, Wang R et al. GARP (LRRC32) is essential for the surface expression of latent TGF-beta on platelets and activated FOXP3+ regulatory T cells. Proc Natl Acad Sci U S A. 2009-08-01 [PMID: 19651619]
• Tran DQ, Shevach EM. Therapeutic potential of FOXP3(+) regulatory T cells and their interactions with dendritic cells. Hum Immunol. 2009-05-01 [PMID: 19236900]

Bioinformatics

• Gene Symbol: LRRC32