LRP-4 Antibody (S164-6)

Western Blot: LRP-4 Antibody (S164-6) [NBP2-22417] - analysis of Rat Brain Membrane showing detection of 215 kDa LRP4 protein using Mouse Anti-LRP4 Monoclonal Antibody, Clone S164-6 . Lane 1: MW Ladder. Lane 2: Rat Brain Membrane (10 ug). . Load: 10 ug. Block: 5% milk. Primary Antibody: Mouse Anti-LRP4 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: 215 kDa.

Immunocytochemistry/Immunofluorescence: LRP-4 Antibody (S164-6) [NBP2-22417] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LRP4 (Cytoplasmic) Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X.

Product Discontinued

LRP-4 Antibody (S164-6) Summary

• Immunogen: Fusion protein amino acids 1747-1905 (cytoplasmic C-terminus) of mouse LRP4
• Localization: Membrane
• Specificity: Detects the cytoplasmic domain of LRP4 approx 215kDa, small degradation products approx 100kD.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LRP4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1ug/ml
• Application Notes: 1 ug/ml of LRP4 (Cytoplasmic) Antibody was sufficient for detection of LRP4in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for LRP-4 Antibody (S164-6)

• CLSS
• KIAA0816
• low density lipoprotein receptor-related protein 4
• low-density lipoprotein receptor-related protein 4
• LRP10
• LRP4
• LRP-4
• MEGF7LRP10
• Multiple epidermal growth factor-like domains 7

Background

Potential cell surface endocytic receptor, which binds and internalizes extracellular ligands for degradation by lysosomes

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LRP4
• Uniprot:
  • Q8VI56()