LRP-1 Antibody (545503) [Unconjugated]

Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Human LRP-1 beta subunit Monoclonal Antibody (Catalog # MAB6360) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LRP-1 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

LRP-1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human LRP-1 beta subunit Monoclonal Antibody (Catalog # MAB6360) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

LRP-1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human LRP-1 beta subunit Monoclonal Antibody (Catalog # MAB6360) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 545503
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

LRP-1 Antibody (545503) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human LRP-1
  Gln4449-ala4544
  Accession # Q07954
• Specificity: Detects human LRP-1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LRP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LRP-1 Antibody (545503) [Unconjugated]

• A2MR
• Alpha-2-Macroglobulin Receptor
• APOER
• Apolipoprotein E receptor
• APR
• CD91 antigen
• CD91
• EC 1.1.1.94
• EC 3.4.21.9
• IGFBP3R
• low density lipoprotein receptor-related protein 1
• LRP
• LRP-1
• LRP1A
• prolow-density lipoprotein receptor-related protein 1
• TbetaR-V/LRP-1/IGFBP-3 receptor
• TGFBR5
• type V tgf-beta receptor

Background

LRP-1 (low-density lipoprotein receptor-related protein 1) is a large type I transmembrane protein belonging to the LDL receptor superfamily. The extracellular domain of LRP-1 is organized in four clusters of ligand-binding repeats that recognizes at least 30 different ligands. The amino acid sequence of human and mouse LRP-1 cluster II share 100% identity. LRP-1 is an endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. It plays a crucial role in early embryonic development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LRP-1 Antibody (MAB6360)(3)

Publications using MAB6360

• N Brookhouse, S Raman, C Frisch, G Srinivasan, DA Brafman APOE2 mitigates disease-related phenotypes in an isogenic hiPSC-based model of Alzheimer's disease Molecular Psychiatry, 2021-04-09;0(0):. 2021-04-09 [PMID: 33837271] (Flow Cytometry, Human)
• R Yang, Y Zhao, Y Gu, Y Yang, X Gao, Y Yuan, L Xiao, J Zhang, C Sun, H Yang, J Qin, J Li, F Zhang, L Zhang, J Ye Isocitrate dehydrogenase 1 mutation enhances 24(S)-hydroxycholesterol production and alters cholesterol homeostasis in glioma Oncogene, 2020-08-27;0(0):. 2020-08-27 [PMID: 32855525] (Western Blot, Mouse)
• Hayashi H, Eguchi Y, Fukuchi-Nakaishi Y, Takeya M, Nakagata N, Tanaka K, Vance JE, Tanihara H A Potential Neuroprotective Role of Apolipoprotein E-containing Lipoproteins through Low Density Lipoprotein Receptor-related Protein 1 in Normal Tension Glaucoma. J. Biol. Chem., 2012-06-06;287(30):25395-406. 2012-06-06 [PMID: 22674573] (Western Blot, Human)

Bioinformatics

• Gene Symbol: LRP1
• Uniprot:
  • Q07954(Human)