LMP7/PSMB8 Antibody

Western Blot: LMP7/PSMB8 Antibody [NBP1-51957] - (0.01ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: LMP7/PSMB8 Antibody [NBP1-51957] - (3.75ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

LMP7/PSMB8 Antibody Summary

• Immunogen: Peptide with sequence C-DVSDLLHQYREANQ corresponding to C-Terminus according to NP_683720.2.
• Epitope: C-DVSDLLHQYREANQ
• Specificity: This antibody is expected to recognize both reported isoforms (NP_004150.1; NP_683720.2).
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PSMB8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.01 - 0.03 ug/ml
• Application Notes: WB: Approx. 22 kDa band observed in human liver, lung and lymph node lysates is reported in scientific literature (PMID: 10878350).Primary incubation was 1 hour.
  IHC: Paraffin embedded Human Spleen.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LMP7/PSMB8 Antibody

• ALDD
• beta5i
• D6S216E
• EC 3.4.25.1
• JMP
• LMP7
• LMP7MGC1491
• Low molecular mass protein 7
• low molecular weight protein 7
• Macropain subunit C13
• Multicatalytic endopeptidase complex subunit C13
• NKJO
• protease component C13
• proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctionalpeptidase 7)
• proteasome catalytic subunit 3i
• Proteasome Component C13
• proteasome subunit beta 5i
• proteasome subunit beta type-8
• Proteasome subunit beta-5i
• Proteasome Subunit Y2
• proteasome-related gene 7
• PSMB5i
• PSMB5iD6S216
• PSMB8
• Really interesting new gene 10 protein
• RING10
• RING10proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctionalprotease 7)
• Y2

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LMP7/PSMB8 Antibody (NBP1-51957)(3)

Publications using NBP1-51957

• Zerfas B, Trader D Synthesis and Application of an Activity Based Peptide Peptoid Hybrid Probe for the Immunoproteasome Current Protocols in Chemical Biology 2019-12-01 [PMID: 31860787] (WB, Human)
• Deshpande A, Wheeler CM, Hunt WC et al. Variation in HLA class I antigen-processing genes and susceptibility to human papillomavirus type 16-associated cervical cancer. J Infect Dis 2008-02-01 [PMID: 18248301]
• Schwarz K, van Den Broek M, Kostka S et al. Overexpression of the proteasome subunits LMP2, LMP7, and MECL-1, but not PA28 alpha/beta, enhances the presentation of an immunodominant lymphocytic choriomeningitis virus T cell epitope. J Immunol 2000-07-01 [PMID: 10878350]

Bioinformatics

• Gene Symbol: PSMB8
• Entrez:
  • 5696(Human)
  • 16913(Mouse)
  • 24968(Rat)
• Uniprot:
  • NP_004150.1(Human)