LIN-41/TRIM71 Antibody

Western Blot: LIN-41/TRIM71 Antibody [NBP2-27490] - Staining of HEK293 (A) and NIH3T3 (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: LIN-41/TRIM71 Antibody [NBP2-27490] - Staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

LIN-41/TRIM71 Antibody Summary

• Description: Novus Biologicals Goat LIN-41/TRIM71 Antibody (NBP2-27490) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-LIN-41/TRIM71 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RQVKAKSLYLQVE corresponding to internal region according to NP_001034200.1.
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRIM71
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 100 kDa band observed in 293 and NIH3T3 lysates (calculated MW of 93.4 kDa band according to human NP_001034200.1and 92.1 kDa band according to mouse NP_001035968.1). The observed molecular weight corresponds to findings with NBP2-27491, a product of different design reacting to a different part of the same protein.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LIN-41/TRIM71 Antibody

• LIN41
• LIN-41
• TRIM71
• tripartite motif containing 71
• tripartite motif-containing protein 71

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIN-41/TRIM71 Antibody (NBP2-27490)(1)

Publication using NBP2-27490

• Lin YC, Hsieh LC, Kuo MW et al. Human TRIM71 and its nematode homologue are targets of let-7 microRNA and its zebrafish orthologue is essential for development. Mol Biol Evol 2007-11-01 [PMID: 17890240]

Bioinformatics

• Gene Symbol: TRIM71
• Entrez:
  • 636331(Mouse)
  • 301042(Rat)
• Uniprot:
  • NP_001034200.1(Human)