Limitin/IFN-zeta Antibody [Unconjugated]

Recombinant Mouse Limitin (1535-LM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (AR002). Inhibition of EMCV activity elicited by Recombinant Mouse Limitin (30 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Limitin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF597). The ND50 is typically 1.5-7.5 ng/mL.

Limitin/IFN‑ zeta was detected in immersion fixed paraffin-embedded sections of mouse thymus using Goat Anti-Mouse Limitin/IFN‑ zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF597) at 1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Limitin/IFN-zeta Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Limitin/IFN‑ zeta
  Leu22-Arg182
  Accession # Q9R1T0
• Specificity: Detects Limitin/IFN‑ zeta in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse IFN-gamma is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Ifnz
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 1.5-7.5 ng/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Limitin/IFN-zeta Antibody [Unconjugated]

• 6030405N23Rik
• BGIF
• Ifnz
• IFN-z
• IFNzeta
• interferon zeta
• Limitin
• Lmtn

Background

Limitin, also known as IFN-zeta , is a 20 kDa glycoprotein that is secreted by T cells and bronchial and salivary duct epithelial cells. It inhibits B-lineage cell development but has little effect on myelopoiesis or erythropoiesis. Limitin binds to IFN-alpha / beta receptors and has potent antiviral activity. Mouse Limitin shares 32%, 26%, and 30% amino acid sequence identity with mouse IFN-alpha , mouse IFN-beta , and human IFN-omega , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Limitin/IFN-zeta Antibody (AF597)(1)

Publication using AF597

• N Bansal, D Kumar, A Gupta, D Chandra, SN Sankhwar, A Mandhani Relevance of MIC-1 in the Era of PSA as a Serum Based Predictor of Prostate Cancer: A Critical Evaluation Sci Rep, 2017-12-04;7(1):16824. 2017-12-04 [PMID: 29203798] (Western Blot, Human)

Bioinformatics

• Gene Symbol: Ifnz
• Uniprot:
  • Q9R1T0()