Limitin/IFN-zeta Products

1535-LM) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (AR002). Inhibition of EMCV activity elicited by Recombinant Mouse Limitin (30 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Limitin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF597). The ND₅₀ is typically 1.5-7.5 ng/mL." class="big_lightbox" target="_blank">

VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox" target="_blank">

1535-LM) was serially diluted and captured by Rat Anti-Mouse Limitin/IFN‑ zeta Monoclonal Antibody (Catalog # MAB5971) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Limitin/IFN‑ zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF597) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">

1535-LM) was serially diluted and captured by Rat Anti-Mouse Limitin/IFN‑ zeta Monoclonal Antibody (Catalog # MAB5971) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Limitin/IFN‑ zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF597) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">