LIMCH1 Antibody - BSA Free

Western Blot: LIMCH1 Antibody [NBP1-32614] - (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with LIMCH1 antibody [C2C3], C-term diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: LIMCH1 Antibody [NBP1-32614] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.

Immunocytochemistry/ Immunofluorescence: LIMCH1 Antibody [NBP1-32614] - LIMCH1 antibody [C2C3], C-term detects LIMCH1 protein at stress fiber by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: LIMCH1 stained by LIMCH1 antibody [C2C3], C-term (NBP1-32614) diluted at 1:500.

Western Blot: LIMCH1 Antibody [NBP1-32614] - Wild-type (WT) and LIMCH1 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with LIMCH1 antibody [C2C3], C-term diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: LIMCH1 Antibody [NBP1-32614] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with LIMCH1 antibody [C2C3], C-term diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

LIMCH1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit LIMCH1 Antibody - BSA Free (NBP1-32614) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human LIMCH1. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LIMCH1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 122 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for LIMCH1 Antibody - BSA Free

• DKFZp434I0312
• DKFZP686A01247
• DKFZp686B2470
• DKFZp686G18243
• DKFZp686G2094
• DKFZp781C1754
• DKFZp781I1455
• KIAA1102
• LIM and calponin homology domains 1
• LIM and calponin homology domains-containing protein 1
• LIMCH1A
• LMO7B
• MGC72127

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: LIMCH1