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LIM domain only 3 Antibody (2H2)

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Immunocytochemistry/Immunofluorescence: LIM domain only 3 Antibody (2H2) [H00055885-M03] - Analysis of monoclonal antibody to LMO3 on HeLa cell. Antibody concentration 10 ug/ml
Immunocytochemistry/ Immunofluorescence: LIM domain only 3 Antibody (2H2) [H00055885-M03] - Analysis of monoclonal antibody to LMO3 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: LIM domain only 3 Antibody (2H2) [H00055885-M03] - Detection limit for recombinant GST tagged LMO3 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
2H2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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LIM domain only 3 Antibody (2H2) Summary

Immunogen
LMO3 (NP_061110, 91 a.a. ~ 146 a.a) partial recombinant protein with GST tag.MRAKDNVYHLDCFACQLCNQRFCVGDKFFLKNNMILCQTDYEEGLMKEGYAPQVR
Specificity
LMO3 - LIM domain only 3 (rhombotin-like 2)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LMO3
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for LIM domain only 3 Antibody (2H2)

  • DAT1
  • LIM domain only 3 (rhombotin-like 2)
  • LMO-3
  • neuronal specific transcription factor DAT1
  • Neuronal-specific transcription factor DAT1
  • RBTN3
  • RBTNL2MGC26081
  • Rhom-3
  • RHOM3LIM domain only protein 3
  • rhombotin-3
  • rhombotin-like 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: LMO1 Antibody (4F7) [H00004004-M01] - Analysis of monoclonal antibody to LMO1 on HeLa cell. Antibody concentration 10 ug/ml.
Species: Hu
Applications: WB, ELISA, ICC/IF
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Western Blot: LMO4 Antibody [NBP2-43538] - Analysis of A. 30 ug 293T whole cell extract B. 30 ug whole cell extract of human LMO4-transfected 293T cells 12 % SDS-PAGE LMO4 antibody dilution: 1:5000.
Immunohistochemistry-Paraffin: LMO4 Antibody [NBP2-43538] - Analysis of human breast carcinoma. LMO4 antibody diluted at 1:500.
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Western Blot: LMO2 Antibody (1A9-3B11) [NB110-78626] - Detection of LMO2 in Ramos cell lysate.
Immunocytochemistry/Immunofluorescence: LMO2 Antibody (1A9-3B11) [NB110-78626] - Immunofluorescent labeling of LMO2 (green) in tonsil tissue.
Immunohistochemistry-Paraffin: LMO2 Antibody (1A9-3B11) [NB110-78626] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using LMO2 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear with some cytoplasmic staining was observed. Staining was performed by Histowiz.
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Western Blot: CSRP3 Antibody [NBP1-06983] - (0.01ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983] - staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Western Blot: PDLIM5 Antibody [NBP1-84843] - Analysis in human cell line A-549.
Immunocytochemistry/Immunofluorescence: PDLIM5 Antibody [NBP1-84843] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & focal adhesion sites.
Immunohistochemistry-Paraffin: PDLIM5 Antibody [NBP1-84843] - Staining of human skeletal muscle shows strong positivity in myocytes.
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Immunocytochemistry/Immunofluorescence: NHLH2 Antibody [NBP2-30642] - Staining of human cell line SiHa shows localization to nucleoplasm & nuclear bodies.
Immunohistochemistry: NHLH2 Antibody [NBP2-30642] - Staining of human kidney shows strong cytoplasmic and membranous positivity in tubule cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
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Western Blot: Slit2 Antibody [NBP2-20398] - Various tissue extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Slit2 Antibody [NBP2-20398] - Paraffin-embedded mouse fetal brain. Green: SLIT2 antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Blue: Nuclear staining with Hoechst 33342.
Western Blot: Slit2 Antibody [NBP2-20398] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SLIT2 antibody diluted at 1:1000.
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Western Blot: Serum Response Factor SRF Antibody [NBP1-33063] - Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE, antibody diluted at 1:500.
Immunocytochemistry/Immunofluorescence: Serum Response Factor SRF Antibody [NBP1-33063] - Paraformaldehyde-fixed A431, using antibody at 1:500 dilution.
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Immunohistochemistry-Paraffin: Reduced Folate Carrier/SLC19A1 Antibody [NBP1-83430] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.
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Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue, loaded at 0.5 mg/mL. A specific band was detected for Aldo‑keto Reductase 1C1/AKR1C1 at approximately 41 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Aldo‑keto Reductase 1C1/AKR1C1 Monoclonal Antibody (Catalog # MAB6529). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Bioinformatics

Gene Symbol LMO3
Entrez
OMIM
Uniprot