LH beta Antibody (LHb/1214)

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Immunohistochemistry-Paraffin: LH beta Antibody (LHb/1214) [NBP2-45130] - Formalin-fixed, paraffin-embedded human Pituitary stained with LH-beta Monoclonal Antibody (LHb/1214).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P
Clone
LHb/1214
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

LH beta Antibody (LHb/1214) Summary

Immunogen
Recombinant beta sub-unit of human LH beta (Uniprot: P01229)
Localization
Cytoplasmic, secreted
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LHB
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47733)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for LH beta Antibody (LHb/1214)

  • CGB4
  • hLHB
  • interstitial cell stimulating hormone, beta chain
  • LH beta
  • LHB
  • LSH-B
  • LSH-beta
  • luteinizing hormone beta polypeptide
  • luteinizing hormone beta subunit
  • lutropin beta chain
  • Lutropin beta
  • lutropin subunit beta

Background

LH is a member of the glycoprotein hormone family. Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.3. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol LHB
Uniprot