Leukotriene B4 R1 Antibody (203/14F11) [Phycoerythrin]

• Applications: Flow
• Clone: 203/14F11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Leukotriene B4 R1 Antibody (203/14F11) [Phycoerythrin] Summary

• Immunogen: HeLa cervical epithelial carcinoma cell line transfected with human Leukotriene B4 R1
• Specificity: Recognizes human Leukotriene B4 R1 (5).
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LTB4R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Polymorphonuclear granulocytes secrete the lipid chemotactic mediator Leukotriene B4 (LTB4) in response to inflammatory stimuli (1). Neutrophils, monocytes and lymphocytes respond to LTB4 via specific receptors localized on the cell surface (2-4). The high affinity LTB4 Receptor known as BLT1 is only expressed on leukocytes (5-7) while a second low affinity receptor BLT2 is expressed more ubiquitously (8, 9). The BLT1 and BLT2 are G-protein linked seven-transmembrane spanning receptors that share about 37-45% amino acid identity (8, 9). Enhanced LTB4 production and engagement of the BLT receptors can be important in allergic and inflammatory diseases such as asthma (10), allergic encephalomyelitis (11), endotoxic shock (12), ischemia (12), psoriasis (13), rheumatoid arthritis (14) and inflammatory bowel disease (15). In addition, it has been reported that BLT1 can function as an additional co-receptor for HIV infection of CD4⁺ T cells (16, 17). Investigations into the mechanisms and potential inhibitors of LTB4 binding to its’ receptors may provide insight into possible treatment modalities for a number of inflammatory disorders.

1. Samuelsson, B. et al. (1987) Science 237:1171.
2. Brom, J. and W. Konig (1989) Immunology 68:479.
3. Patry, C. et al. (1996) Prostaglandins, Leukotrienes and Essential Fatty Acids 54:361.
4. Dasari, V.R. et al. (2000) Immunopharm. 48:157.
5. Pettersson, A. et al. (2000) Biochem. Biophys. Res. Comm. 279:520.
6. Yokomizo, T. et al. (1997) Nature 387:620.
7. Kato, K. et al. (2000) J. Exp. Med. 192:413.
8. Yokomizo, T. et al. (2000) J. Exp. Med. 192:421.
9. Kamohara, M. et al. (2000) J. Biol. Chem. 275:27000.
10. Turner, C.R. et al. (1996) J. Clin. Invest. 97:381.
11. Gladue, R.P. et al. (1996) J. Exp. Med. 183:1893.
12. Lefer, A.M. (1986) Biochem. Pharmacol. 35:123.
13. Iverson, L. et al. (1997) Skin Pharmacol. 10:169.
14. Griffiths, R.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:517.
15. Sharon, P. and W.F. Stenson (1984) Gastroenterology 86:453.
16. Owman, C. et al. (1996) Proc. Natl. Acad. Sci. USA 95:9530.
17. Martin, V. et al. (1999) J. Biol. Chem. 274:8597.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LTB4R