LEPRE1 Antibody (3C7) - Azide and BSA Free

Western Blot: LEPRE1 Antibody (3C7) [H00064175-M01] - LEPRE1 monoclonal antibody (M01), clone 3C7 Analysis of LEPRE1 expression in HeLa.

Immunocytochemistry/Immunofluorescence: LEPRE1 Antibody (3C7) [H00064175-M01] - Analysis of monoclonal antibody to LEPRE1 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: LEPRE1 Antibody (3C7) [H00064175-M01] - Analysis of monoclonal antibody to LEPRE1 on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 6 ug/ml.

Western Blot: LEPRE1 Antibody (3C7) [H00064175-M01] - Analysis of LEPRE1 expression in transfected 293T cell line by LEPRE1 monoclonal antibody (M01), clone 3C7.Lane 1: LEPRE1 transfected lysate(46 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: LEPRE1 Antibody (3C7) [H00064175-M01] - Detection limit for recombinant GST tagged LEPRE1 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3C7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

LEPRE1 Antibody (3C7) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse LEPRE1 Antibody (3C7) - Azide and BSA Free (H00064175-M01) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-LEPRE1 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: LEPRE1 (AAH15309, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DPRVREVMNQNLAYYAAMLGEEHTRSIGPRESAKEYRQRSLLEKELLFFAYDVFGIPFVDPDSWTPEEVIPKRLQEKQKSERETAVRISQEIGNLMKEIE
• Specificity: LEPRE1 - leucine proline-enriched proteoglycan (leprecan) 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: P3H1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recominant protein, transfected lysate and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA. Reactivity with Mouse species is reported in scientific literature (PMID 19997487)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for LEPRE1 Antibody (3C7) - Azide and BSA Free

• EC 1.14.11.7
• GROS1OI8
• Growth suppressor 1
• leprecan
• Leprecan-1
• Leucine- and proline-enriched proteoglycan 1
• leucine proline-enriched proteoglycan (leprecan) 1
• MGC117314
• P3H1LEPRECAN
• prolyl 3-hydroxylase 1

Background

This gene encodes an enzyme that is a member of the collagen prolyl hydroxylase family. These enzymes are localized to the endoplasmic reticulum and their activity is required for proper collagen synthesis and assembly. Mutations in this gene are associated with osteogenesis imperfecta type VIII. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LEPRE1 Antibody (H00064175-M01)(5)

Publications using H00064175-M01

• Zimmerman SM, Besio R, Heard-Lipsmeyer ME et al. Expression characterization and functional implication of the collagen-modifying Leprecan proteins in mouse gonadal tissue and mature sperm. AIMS Genet 2018-02-07 [PMID: 30417103]
• Ohe R, Aung NY, Meng H et al. Localization of collagen modifying enzymes on fibroblastic reticular cells and follicular dendritic cells in non-neoplastic and neoplastic lymphoid tissues. Leuk Lymphoma 2015-12-01 [PMID: 26700650]
• Lai Y, Song M, Hakala K et al. Proteomic dissection of the VHL interactome. J Proteome Res. 2011-10-11 [PMID: 21942715]
• Choi JW, Sutor SL, Lindquist L et al. Severe osteogenesis imperfecta in cyclophilin B-deficient mice. PLoS Genet 5(12):e1000750. 2009-12-01 [PMID: 19997487]
• Cabral WA, Chang W, Barnes AM et al. Prolyl 3-hydroxylase 1 deficiency causes a recessive metabolic bone disorder resembling lethal/severe osteogenesis imperfecta. Nat Genet;39(3):359-65. 2007-03-01 [PMID: 17277775]

Bioinformatics

• Gene Symbol: P3H1
• Entrez:
  • 64175(Human)
• Uniprot:
  • AAH15309(Human)