LATS1 293T Cell Transient Overexpression Lysate

Product Discontinued

LATS1 293T Cell Transient Overexpression Lysate Summary

• Description: Quality control test: Transient overexpression cell lysate was tested with Anti-LATS1 antibody by Western Blots. Plasmid: pCMV-LATS1 full-length
• Specificity: LATS1 293T Cell Transient Overexpression Lysate(Denatured)
• Gene: LATS1

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

• Type: 293T Cell Transient Overexpression
• Protein State: Denatured

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for LATS1 293T Cell Transient Overexpression Lysate

• EC 2.7.11
• h-warts
• Large tumor suppressor homolog 1
• LATS (large tumor suppressor, Drosophila) homolog 1
• LATS, large tumor suppressor, homolog 1 (Drosophila)
• serine/threonine-protein kinase LATS1
• WARTS protein kinase
• WARTSEC 2.7.11.1
• wts

Background

LATS1 is encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. In addition, the C-terminal kinase domain binds to its own N-terminal region, suggesting potential negative regulation through interference with complex formation via intramolecular binding. Biochemical and genetic data suggest a role as a tumor suppressor. This is supported by studies in knockout mice showing development of soft-tissue sarcomas, ovarian stromal cell tumors and a high sensitivity to carcinogenic treatments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: LATS1